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An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313664/ https://www.ncbi.nlm.nih.gov/pubmed/34312441 http://dx.doi.org/10.1038/s41598-021-94652-0 |
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author | Deng, Hao Jayawardena, Asanka Chan, Jianxiong Tan, Sher Maine Alan, Tuncay Kwan, Patrick |
author_facet | Deng, Hao Jayawardena, Asanka Chan, Jianxiong Tan, Sher Maine Alan, Tuncay Kwan, Patrick |
author_sort | Deng, Hao |
collection | PubMed |
description | There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm(3)) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions. |
format | Online Article Text |
id | pubmed-8313664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83136642021-07-28 An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection Deng, Hao Jayawardena, Asanka Chan, Jianxiong Tan, Sher Maine Alan, Tuncay Kwan, Patrick Sci Rep Article There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm(3)) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions. Nature Publishing Group UK 2021-07-26 /pmc/articles/PMC8313664/ /pubmed/34312441 http://dx.doi.org/10.1038/s41598-021-94652-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Deng, Hao Jayawardena, Asanka Chan, Jianxiong Tan, Sher Maine Alan, Tuncay Kwan, Patrick An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title | An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title_full | An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title_fullStr | An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title_full_unstemmed | An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title_short | An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection |
title_sort | ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active covid-19 infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313664/ https://www.ncbi.nlm.nih.gov/pubmed/34312441 http://dx.doi.org/10.1038/s41598-021-94652-0 |
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