Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells

Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Peng, Ren, Lejiao, Zhi, Li, Hu, Xinli, Xiao, Rui-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313743/
https://www.ncbi.nlm.nih.gov/pubmed/34337441
http://dx.doi.org/10.1016/j.xpro.2021.100497
Descripción
Sumario:Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for the downstream functional analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

Ejemplares similares