Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells

Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro...

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Autores principales: Jiang, Peng, Ren, Lejiao, Zhi, Li, Hu, Xinli, Xiao, Rui-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313743/
https://www.ncbi.nlm.nih.gov/pubmed/34337441
http://dx.doi.org/10.1016/j.xpro.2021.100497
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author Jiang, Peng
Ren, Lejiao
Zhi, Li
Hu, Xinli
Xiao, Rui-Ping
author_facet Jiang, Peng
Ren, Lejiao
Zhi, Li
Hu, Xinli
Xiao, Rui-Ping
author_sort Jiang, Peng
collection PubMed
description Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for the downstream functional analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).
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spelling pubmed-83137432021-07-31 Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells Jiang, Peng Ren, Lejiao Zhi, Li Hu, Xinli Xiao, Rui-Ping STAR Protoc Protocol Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for the downstream functional analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021). Elsevier 2021-07-17 /pmc/articles/PMC8313743/ /pubmed/34337441 http://dx.doi.org/10.1016/j.xpro.2021.100497 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jiang, Peng
Ren, Lejiao
Zhi, Li
Hu, Xinli
Xiao, Rui-Ping
Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title_full Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title_fullStr Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title_full_unstemmed Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title_short Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
title_sort protocol for cell preparation and gene delivery in hek293t and c2c12 cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313743/
https://www.ncbi.nlm.nih.gov/pubmed/34337441
http://dx.doi.org/10.1016/j.xpro.2021.100497
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