Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake

The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a p...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Peng, Zhi, Li, Ren, Lejiao, Hu, Xinli, Xiao, Rui-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313746/
https://www.ncbi.nlm.nih.gov/pubmed/34337440
http://dx.doi.org/10.1016/j.xpro.2021.100496
_version_ 1783729409406009344
author Jiang, Peng
Zhi, Li
Ren, Lejiao
Hu, Xinli
Xiao, Rui-Ping
author_facet Jiang, Peng
Zhi, Li
Ren, Lejiao
Hu, Xinli
Xiao, Rui-Ping
author_sort Jiang, Peng
collection PubMed
description The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a protocol accessing AMPK activity in mice by measuring glucose uptake induced by AMPK activators, MK8722 and A769662. This protocol can be used to evaluate AMPK signaling in vivo under various pathophysiological conditions. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).
format Online
Article
Text
id pubmed-8313746
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83137462021-07-31 Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake Jiang, Peng Zhi, Li Ren, Lejiao Hu, Xinli Xiao, Rui-Ping STAR Protoc Protocol The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a protocol accessing AMPK activity in mice by measuring glucose uptake induced by AMPK activators, MK8722 and A769662. This protocol can be used to evaluate AMPK signaling in vivo under various pathophysiological conditions. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021). Elsevier 2021-07-17 /pmc/articles/PMC8313746/ /pubmed/34337440 http://dx.doi.org/10.1016/j.xpro.2021.100496 Text en © 2021. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jiang, Peng
Zhi, Li
Ren, Lejiao
Hu, Xinli
Xiao, Rui-Ping
Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title_full Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title_fullStr Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title_full_unstemmed Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title_short Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake
title_sort evaluation of ampk activity in mice by measuring activator-induced glucose uptake
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313746/
https://www.ncbi.nlm.nih.gov/pubmed/34337440
http://dx.doi.org/10.1016/j.xpro.2021.100496
work_keys_str_mv AT jiangpeng evaluationofampkactivityinmicebymeasuringactivatorinducedglucoseuptake
AT zhili evaluationofampkactivityinmicebymeasuringactivatorinducedglucoseuptake
AT renlejiao evaluationofampkactivityinmicebymeasuringactivatorinducedglucoseuptake
AT huxinli evaluationofampkactivityinmicebymeasuringactivatorinducedglucoseuptake
AT xiaoruiping evaluationofampkactivityinmicebymeasuringactivatorinducedglucoseuptake

Ejemplares similares