Cargando…
A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy
Ubiquitin stress-induced NEDDylation leads to the formation of aggresome-like bodies (ALBs) in the perinuclear region of cells. Therefore, imaging analysis is essential for characterizing the biological phenotypes of ALBs. Here, we describe a protocol to monitor ALBs induced by ubiquitin stress usin...
Autores principales: | Kim, Soyeon, Hwang, Yiseul, Kang, Ho Chul |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313748/ https://www.ncbi.nlm.nih.gov/pubmed/34337443 http://dx.doi.org/10.1016/j.xpro.2021.100674 |
Ejemplares similares
-
Fast in vitro protocol for the visualization and quantitative high-throughput analysis of sprouting angiogenesis by confocal microscopy
por: Kempers, Lanette, et al.
Publicado: (2021) -
Stress-induced NEDDylation promotes cytosolic protein aggregation through HDAC6 in a p62-dependent manner
por: Kim, Soyeon, et al.
Publicado: (2021) -
Characterization and Dynamics of Aggresome Formation by a Cytosolic Gfp-Chimera✪
por: García-Mata, Rafael, et al.
Publicado: (1999) -
Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy
por: Guo, Xiaoli, et al.
Publicado: (2022) -
Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy
por: Ji, Wangsheng, et al.
Publicado: (2023)