Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing

Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an inte...

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Detalles Bibliográficos
Autores principales: Cheng, Shanshan, Shen, Yuqian, Zhang, Zi Chao, Han, Junhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313749/
https://www.ncbi.nlm.nih.gov/pubmed/34337444
http://dx.doi.org/10.1016/j.xpro.2021.100679
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author Cheng, Shanshan
Shen, Yuqian
Zhang, Zi Chao
Han, Junhai
author_facet Cheng, Shanshan
Shen, Yuqian
Zhang, Zi Chao
Han, Junhai
author_sort Cheng, Shanshan
collection PubMed
description Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippocampal mGluR-LTD and mGluR-LTD-associated behaviors. For complete details on the use and execution of this protocol, please refer to Shen et al. (2021).
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spelling pubmed-83137492021-07-31 Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing Cheng, Shanshan Shen, Yuqian Zhang, Zi Chao Han, Junhai STAR Protoc Protocol Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippocampal mGluR-LTD and mGluR-LTD-associated behaviors. For complete details on the use and execution of this protocol, please refer to Shen et al. (2021). Elsevier 2021-07-17 /pmc/articles/PMC8313749/ /pubmed/34337444 http://dx.doi.org/10.1016/j.xpro.2021.100679 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Cheng, Shanshan
Shen, Yuqian
Zhang, Zi Chao
Han, Junhai
Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title_full Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title_fullStr Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title_full_unstemmed Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title_short Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
title_sort protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313749/
https://www.ncbi.nlm.nih.gov/pubmed/34337444
http://dx.doi.org/10.1016/j.xpro.2021.100679
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