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The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture

Among the terrestrial Crustacea, isopods have most successfully established themselves in a large variety of terrestrial habitats. As in most Crustacea, their cuticle consists of a hierarchically organised organic phase of chitin-protein fibrils, containing calcium carbonate and some calcium phospha...

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Autores principales: Seidl, Bastian, Reisecker, Christian, Neues, Frank, Campanaro, Alessandro, Epple, Matthias, Hild, Sabine, Ziegler, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313847/
https://www.ncbi.nlm.nih.gov/pubmed/34337383
http://dx.doi.org/10.1016/j.yjsbx.2021.100051
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author Seidl, Bastian
Reisecker, Christian
Neues, Frank
Campanaro, Alessandro
Epple, Matthias
Hild, Sabine
Ziegler, Andreas
author_facet Seidl, Bastian
Reisecker, Christian
Neues, Frank
Campanaro, Alessandro
Epple, Matthias
Hild, Sabine
Ziegler, Andreas
author_sort Seidl, Bastian
collection PubMed
description Among the terrestrial Crustacea, isopods have most successfully established themselves in a large variety of terrestrial habitats. As in most Crustacea, their cuticle consists of a hierarchically organised organic phase of chitin-protein fibrils, containing calcium carbonate and some calcium phosphate. In previous studies, we examined the tergite cuticle of Tylos europaeus, which lives on seashores and burrows into moist sand. In this study, we investigate the closely related species Helleria brevicornis, which is completely terrestrial and lives in leaf litter and humus and burrows into the soil. To get deeper insights in relation between the structure of the organic and mineral phase in species living in diverse habitats, we have investigated the structure, and the chemical and crystallographic properties of the tergite cuticle using various preparation techniques, and microscopic and analytical methods. The results reveal long and short epicuticular sensilla with brushed tips on the tergite surface that do not occur in T. europaeus. As in T. europaeus a distal exocuticle, which contains a low number of organic fibres, contains calcite while the subjacent layers of the exo- and endocuticle contain amorphous calcium carbonate. The distal exocuticle contains a polygonal pattern of mineral initiation sites that correspond to interprismatic septa described for decapod crabs. The shape and position of calcite units do not follow the polygonal pattern of the septa. The results indicate that the calcite units form by crystallisation from an amorphous phase that progresses from both margins of the septa to the centres of the polygons.
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spelling pubmed-83138472021-07-31 The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture Seidl, Bastian Reisecker, Christian Neues, Frank Campanaro, Alessandro Epple, Matthias Hild, Sabine Ziegler, Andreas J Struct Biol X Article Among the terrestrial Crustacea, isopods have most successfully established themselves in a large variety of terrestrial habitats. As in most Crustacea, their cuticle consists of a hierarchically organised organic phase of chitin-protein fibrils, containing calcium carbonate and some calcium phosphate. In previous studies, we examined the tergite cuticle of Tylos europaeus, which lives on seashores and burrows into moist sand. In this study, we investigate the closely related species Helleria brevicornis, which is completely terrestrial and lives in leaf litter and humus and burrows into the soil. To get deeper insights in relation between the structure of the organic and mineral phase in species living in diverse habitats, we have investigated the structure, and the chemical and crystallographic properties of the tergite cuticle using various preparation techniques, and microscopic and analytical methods. The results reveal long and short epicuticular sensilla with brushed tips on the tergite surface that do not occur in T. europaeus. As in T. europaeus a distal exocuticle, which contains a low number of organic fibres, contains calcite while the subjacent layers of the exo- and endocuticle contain amorphous calcium carbonate. The distal exocuticle contains a polygonal pattern of mineral initiation sites that correspond to interprismatic septa described for decapod crabs. The shape and position of calcite units do not follow the polygonal pattern of the septa. The results indicate that the calcite units form by crystallisation from an amorphous phase that progresses from both margins of the septa to the centres of the polygons. Elsevier 2021-07-10 /pmc/articles/PMC8313847/ /pubmed/34337383 http://dx.doi.org/10.1016/j.yjsbx.2021.100051 Text en © 2021 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Seidl, Bastian
Reisecker, Christian
Neues, Frank
Campanaro, Alessandro
Epple, Matthias
Hild, Sabine
Ziegler, Andreas
The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title_full The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title_fullStr The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title_full_unstemmed The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title_short The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture
title_sort dorsal tergite cuticle of helleria brevicornis: ultrastructure, mineral distribution, calcite microstructure and texture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313847/
https://www.ncbi.nlm.nih.gov/pubmed/34337383
http://dx.doi.org/10.1016/j.yjsbx.2021.100051
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