Cargando…

Reconstitution of Pol II (G) responsive form of the human Mediator complex

RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dep...

Descripción completa

Detalles Bibliográficos
Autor principal: CEVHER, Murat Alper
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313941/
https://www.ncbi.nlm.nih.gov/pubmed/34377050
http://dx.doi.org/10.3906/biy-2009-12
_version_ 1783729450480828416
author CEVHER, Murat Alper
author_facet CEVHER, Murat Alper
author_sort CEVHER, Murat Alper
collection PubMed
description RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription both with full 30-subunit Mediator and also with 15-subunit recombinant Mediator that we synthesized. Moreover, we also show that the recombinant Mediator interacts with Pol II (G) as well. These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex.
format Online
Article
Text
id pubmed-8313941
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Scientific and Technological Research Council of Turkey
record_format MEDLINE/PubMed
spelling pubmed-83139412021-08-09 Reconstitution of Pol II (G) responsive form of the human Mediator complex CEVHER, Murat Alper Turk J Biol Article RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription both with full 30-subunit Mediator and also with 15-subunit recombinant Mediator that we synthesized. Moreover, we also show that the recombinant Mediator interacts with Pol II (G) as well. These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex. The Scientific and Technological Research Council of Turkey 2021-06-23 /pmc/articles/PMC8313941/ /pubmed/34377050 http://dx.doi.org/10.3906/biy-2009-12 Text en Copyright © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
CEVHER, Murat Alper
Reconstitution of Pol II (G) responsive form of the human Mediator complex
title Reconstitution of Pol II (G) responsive form of the human Mediator complex
title_full Reconstitution of Pol II (G) responsive form of the human Mediator complex
title_fullStr Reconstitution of Pol II (G) responsive form of the human Mediator complex
title_full_unstemmed Reconstitution of Pol II (G) responsive form of the human Mediator complex
title_short Reconstitution of Pol II (G) responsive form of the human Mediator complex
title_sort reconstitution of pol ii (g) responsive form of the human mediator complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313941/
https://www.ncbi.nlm.nih.gov/pubmed/34377050
http://dx.doi.org/10.3906/biy-2009-12
work_keys_str_mv AT cevhermuratalper reconstitutionofpoliigresponsiveformofthehumanmediatorcomplex