Cargando…

Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks

The present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, Lin, Qian, Huang, Xuan, Jiang, Guitao, Li, Chuang, Zhang, Xu, Liu, Shengli, He, Lingyun, Liu, Yali, Dai, Qiuzhong, Huang, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313987/
https://www.ncbi.nlm.nih.gov/pubmed/34326826
http://dx.doi.org/10.3389/fmicb.2021.698213
_version_ 1783729456845684736
author Liu, Yang
Lin, Qian
Huang, Xuan
Jiang, Guitao
Li, Chuang
Zhang, Xu
Liu, Shengli
He, Lingyun
Liu, Yali
Dai, Qiuzhong
Huang, Xingguo
author_facet Liu, Yang
Lin, Qian
Huang, Xuan
Jiang, Guitao
Li, Chuang
Zhang, Xu
Liu, Shengli
He, Lingyun
Liu, Yali
Dai, Qiuzhong
Huang, Xingguo
author_sort Liu, Yang
collection PubMed
description The present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups. Each group contained six replicates of 10 birds. The dietary treatments were corn-soybean-based diet supplemented with FA at the concentrations of 0 (control), 100, 200, 400, and 800 mg/kg diet. The results demonstrated that dietary FA at the levels of 200, 400, and 800 mg/kg increased the average daily gain (P = 0.01), 400 and 800 mg/kg FA increased the final body weight (P = 0.02), 100, 200, and 800 mg/kg FA increased the serum glutathione (P = 0.01), and 100, 400, and 800 mg/kg FA increased the glutathione peroxidase activities in birds (P < 0.01). Additionally, 200, 400, and 800 mg/kg dietary FA lowered the serum levels of interleukin-2 (P = 0.02) and interleukin-6 (P = 0.04). Moreover, the morphometric study of the intestines indicated that 400 mg/kg FA decreased the crypt depth in jejunum (P = 0.01) and caecum (P = 0.04), and increased the ratio of villus height to crypt depth in jejunum (P = 0.02). Significant linear and/or quadratic relationships were found between FA concentration and the measured parameters. 16S rRNA sequencing revealed that dietary FA increased the populations of genera Faecalibacterium, Paludicola, RF39, and Faecalicoccus in the cecum (P < 0.05), whereas decreased the populations of Anaerofilum and UCG-002 (P < 0.05). The Spearman correlation analysis indicated that phylum Proteobacteria were negatively, but order Oscillospirales, and family Ruminococcaceae were positively related to the parameters of the growth performance. Phylum Bacteroidetes, class Negativicutes and family Rikenellaceae were negatively associated with the parameters of the antioxidative capability. And phylum Cyanobacteria, Elusimicrobia, and Bacteroidetes, class Bacilli, family Rikenellaceae, and genus Prevotella were positively associated with the parameters of the immunological capability. Thus, it was concluded that the supplementations of 400 mg/kg FA in diet was able to improve the growth performance, antioxidative and immunological capabilities, intestinal morphology, and modulated the gut microbial construction of Linwu ducks at the growing stage.
format Online
Article
Text
id pubmed-8313987
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-83139872021-07-28 Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks Liu, Yang Lin, Qian Huang, Xuan Jiang, Guitao Li, Chuang Zhang, Xu Liu, Shengli He, Lingyun Liu, Yali Dai, Qiuzhong Huang, Xingguo Front Microbiol Microbiology The present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups. Each group contained six replicates of 10 birds. The dietary treatments were corn-soybean-based diet supplemented with FA at the concentrations of 0 (control), 100, 200, 400, and 800 mg/kg diet. The results demonstrated that dietary FA at the levels of 200, 400, and 800 mg/kg increased the average daily gain (P = 0.01), 400 and 800 mg/kg FA increased the final body weight (P = 0.02), 100, 200, and 800 mg/kg FA increased the serum glutathione (P = 0.01), and 100, 400, and 800 mg/kg FA increased the glutathione peroxidase activities in birds (P < 0.01). Additionally, 200, 400, and 800 mg/kg dietary FA lowered the serum levels of interleukin-2 (P = 0.02) and interleukin-6 (P = 0.04). Moreover, the morphometric study of the intestines indicated that 400 mg/kg FA decreased the crypt depth in jejunum (P = 0.01) and caecum (P = 0.04), and increased the ratio of villus height to crypt depth in jejunum (P = 0.02). Significant linear and/or quadratic relationships were found between FA concentration and the measured parameters. 16S rRNA sequencing revealed that dietary FA increased the populations of genera Faecalibacterium, Paludicola, RF39, and Faecalicoccus in the cecum (P < 0.05), whereas decreased the populations of Anaerofilum and UCG-002 (P < 0.05). The Spearman correlation analysis indicated that phylum Proteobacteria were negatively, but order Oscillospirales, and family Ruminococcaceae were positively related to the parameters of the growth performance. Phylum Bacteroidetes, class Negativicutes and family Rikenellaceae were negatively associated with the parameters of the antioxidative capability. And phylum Cyanobacteria, Elusimicrobia, and Bacteroidetes, class Bacilli, family Rikenellaceae, and genus Prevotella were positively associated with the parameters of the immunological capability. Thus, it was concluded that the supplementations of 400 mg/kg FA in diet was able to improve the growth performance, antioxidative and immunological capabilities, intestinal morphology, and modulated the gut microbial construction of Linwu ducks at the growing stage. Frontiers Media S.A. 2021-07-13 /pmc/articles/PMC8313987/ /pubmed/34326826 http://dx.doi.org/10.3389/fmicb.2021.698213 Text en Copyright © 2021 Liu, Lin, Huang, Jiang, Li, Zhang, Liu, He, Liu, Dai and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yang
Lin, Qian
Huang, Xuan
Jiang, Guitao
Li, Chuang
Zhang, Xu
Liu, Shengli
He, Lingyun
Liu, Yali
Dai, Qiuzhong
Huang, Xingguo
Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title_full Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title_fullStr Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title_full_unstemmed Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title_short Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
title_sort effects of dietary ferulic acid on the intestinal microbiota and the associated changes on the growth performance, serum cytokine profile, and intestinal morphology in ducks
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313987/
https://www.ncbi.nlm.nih.gov/pubmed/34326826
http://dx.doi.org/10.3389/fmicb.2021.698213
work_keys_str_mv AT liuyang effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT linqian effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT huangxuan effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT jiangguitao effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT lichuang effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT zhangxu effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT liushengli effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT helingyun effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT liuyali effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT daiqiuzhong effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks
AT huangxingguo effectsofdietaryferulicacidontheintestinalmicrobiotaandtheassociatedchangesonthegrowthperformanceserumcytokineprofileandintestinalmorphologyinducks