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De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes

Polysaccharides separated from Lentinula edodes are well known for their medicinal properties. However, the precise molecular mechanisms of polysaccharide biosynthesis in L. edodes remain unclear. In this study, the fruiting bodies of L. edodes in four developmental stages with significant differenc...

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Autores principales: Li, Qiaozhen, Chen, Jing, Liu, Jianyu, Yu, Hailong, Zhang, Lujun, Song, Chunyan, Li, Yu, Jiang, Ning, Tan, Qi, Shang, Xiaodong, Gu, Yunfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313990/
https://www.ncbi.nlm.nih.gov/pubmed/34326817
http://dx.doi.org/10.3389/fmicb.2021.627099
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author Li, Qiaozhen
Chen, Jing
Liu, Jianyu
Yu, Hailong
Zhang, Lujun
Song, Chunyan
Li, Yu
Jiang, Ning
Tan, Qi
Shang, Xiaodong
Gu, Yunfu
author_facet Li, Qiaozhen
Chen, Jing
Liu, Jianyu
Yu, Hailong
Zhang, Lujun
Song, Chunyan
Li, Yu
Jiang, Ning
Tan, Qi
Shang, Xiaodong
Gu, Yunfu
author_sort Li, Qiaozhen
collection PubMed
description Polysaccharides separated from Lentinula edodes are well known for their medicinal properties. However, the precise molecular mechanisms of polysaccharide biosynthesis in L. edodes remain unclear. In this study, the fruiting bodies of L. edodes in four developmental stages with significant differences in polysaccharide yield were collected, and the characteristics of polysaccharides were studied. De novo sequencing and comparative transcriptomic analysis were performed by using high-throughput Illumina RNA-sequencing. KS1P30, KS2P30, KS3P30, and KS4P30 were obtained from the four developmental stages, respectively, by hot water extraction and 30% ethanol precipitation. These four polysaccharides had good immune activity in vitro; all of them were β-glucopyranose with a high molecular weight. Glucose was the main monosaccharide component of these polysaccharides. High-quality clean reads (57.88, 53.17, 53.28, and 47.56 million for different growth stages) and mapping ratios ranging from 84.75 to 90.11% were obtained. In total, 11,493 (96.56%) unigenes and 18,924 (97.46%) transcripts were successfully annotated in five public databases. The biosynthetic pathway and related genes of LEFP30 were mined. The molecular mechanism of LEFP30 yield change in the different developmental stages was predicted. The results provide some insights into the possible mechanisms involved in the biosynthetic pathway of this kind of polysaccharide in L. edodes fruiting bodies. They also indicate that candidate genes can be used as important resources for biotechnology and molecular breeding to regulate L. edodes fruiting body polysaccharide biosynthesis.
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spelling pubmed-83139902021-07-28 De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes Li, Qiaozhen Chen, Jing Liu, Jianyu Yu, Hailong Zhang, Lujun Song, Chunyan Li, Yu Jiang, Ning Tan, Qi Shang, Xiaodong Gu, Yunfu Front Microbiol Microbiology Polysaccharides separated from Lentinula edodes are well known for their medicinal properties. However, the precise molecular mechanisms of polysaccharide biosynthesis in L. edodes remain unclear. In this study, the fruiting bodies of L. edodes in four developmental stages with significant differences in polysaccharide yield were collected, and the characteristics of polysaccharides were studied. De novo sequencing and comparative transcriptomic analysis were performed by using high-throughput Illumina RNA-sequencing. KS1P30, KS2P30, KS3P30, and KS4P30 were obtained from the four developmental stages, respectively, by hot water extraction and 30% ethanol precipitation. These four polysaccharides had good immune activity in vitro; all of them were β-glucopyranose with a high molecular weight. Glucose was the main monosaccharide component of these polysaccharides. High-quality clean reads (57.88, 53.17, 53.28, and 47.56 million for different growth stages) and mapping ratios ranging from 84.75 to 90.11% were obtained. In total, 11,493 (96.56%) unigenes and 18,924 (97.46%) transcripts were successfully annotated in five public databases. The biosynthetic pathway and related genes of LEFP30 were mined. The molecular mechanism of LEFP30 yield change in the different developmental stages was predicted. The results provide some insights into the possible mechanisms involved in the biosynthetic pathway of this kind of polysaccharide in L. edodes fruiting bodies. They also indicate that candidate genes can be used as important resources for biotechnology and molecular breeding to regulate L. edodes fruiting body polysaccharide biosynthesis. Frontiers Media S.A. 2021-07-13 /pmc/articles/PMC8313990/ /pubmed/34326817 http://dx.doi.org/10.3389/fmicb.2021.627099 Text en Copyright © 2021 Li, Chen, Liu, Yu, Zhang, Song, Li, Jiang, Tan, Shang and Gu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Qiaozhen
Chen, Jing
Liu, Jianyu
Yu, Hailong
Zhang, Lujun
Song, Chunyan
Li, Yu
Jiang, Ning
Tan, Qi
Shang, Xiaodong
Gu, Yunfu
De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title_full De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title_fullStr De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title_full_unstemmed De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title_short De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
title_sort de novo sequencing and comparative transcriptome analyses provide first insights into polysaccharide biosynthesis during fruiting body development of lentinula edodes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313990/
https://www.ncbi.nlm.nih.gov/pubmed/34326817
http://dx.doi.org/10.3389/fmicb.2021.627099
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