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Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma

INTRODUCTION: Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA) implicated in cell longevity and apoptosis. We aimed in the current work for the first time to investigate the association of the expression profiles of...

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Autores principales: Fawzy, Manal S., Toraih, Eman A., El-Wazir, Aya, Hosny, Marwa M., Badran, Dahlia I., El Kelish, Amr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314397/
https://www.ncbi.nlm.nih.gov/pubmed/34336029
http://dx.doi.org/10.5114/aoms.2019.85201
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author Fawzy, Manal S.
Toraih, Eman A.
El-Wazir, Aya
Hosny, Marwa M.
Badran, Dahlia I.
El Kelish, Amr
author_facet Fawzy, Manal S.
Toraih, Eman A.
El-Wazir, Aya
Hosny, Marwa M.
Badran, Dahlia I.
El Kelish, Amr
author_sort Fawzy, Manal S.
collection PubMed
description INTRODUCTION: Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA) implicated in cell longevity and apoptosis. We aimed in the current work for the first time to investigate the association of the expression profiles of LINC-ROR and three stem-related transcriptional factors with clinicopathological data and their impact on renal cell carcinoma (RCC) progression in a sample of RCC patients. MATERIAL AND METHODS: Expression levels of LINC-ROR and stemness-related factors: SOX2, NANOG, and POU5F1 were detected in 60 formalin-fixed, paraffin-embedded tissues, and their paired adjacent non-cancer tissues (n = 60) by using real-time qRT-PCR analysis. Additionally, the expression profiles were compared with the available clinicopathological features. RESULTS: The genes studied were markedly up-regulated in RCC (medians and interquartile ranges were 30.3 (1.84–235.5), 10.2 (1.84–53.9), 5.39 (0.94–23.5), and 12.5 (1.61–43.2) for LINC-ROR, SOX2, NANOG, and POU5F1, respectively) relative to paired non-cancer tissue. High expression levels were associated with poor prognosis in terms of tumour undifferentiation (for LINC-ROR, SOX2, and NANOG), lymph node infiltration (for SOX2), postoperative recurrence (for LINC-ROR and SOX2), and shorter overall survival (OS) and progression-free survival (for all genes studied). The best curve for OS prediction was constructed with LINC-ROR data (area under the receiver operating characteristic curve (AUC) = 0.804 at a cut-off value of 72.7, sensitivity 78.9%, and specificity 80.5%). CONCLUSIONS: Collectively, aberrant LINC-ROR and pluripotent gene expression may be recognised as prognostic markers for RCC. Future functional studies are highly recommended to validate the study findings.
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spelling pubmed-83143972021-07-31 Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma Fawzy, Manal S. Toraih, Eman A. El-Wazir, Aya Hosny, Marwa M. Badran, Dahlia I. El Kelish, Amr Arch Med Sci Basic Research INTRODUCTION: Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA) implicated in cell longevity and apoptosis. We aimed in the current work for the first time to investigate the association of the expression profiles of LINC-ROR and three stem-related transcriptional factors with clinicopathological data and their impact on renal cell carcinoma (RCC) progression in a sample of RCC patients. MATERIAL AND METHODS: Expression levels of LINC-ROR and stemness-related factors: SOX2, NANOG, and POU5F1 were detected in 60 formalin-fixed, paraffin-embedded tissues, and their paired adjacent non-cancer tissues (n = 60) by using real-time qRT-PCR analysis. Additionally, the expression profiles were compared with the available clinicopathological features. RESULTS: The genes studied were markedly up-regulated in RCC (medians and interquartile ranges were 30.3 (1.84–235.5), 10.2 (1.84–53.9), 5.39 (0.94–23.5), and 12.5 (1.61–43.2) for LINC-ROR, SOX2, NANOG, and POU5F1, respectively) relative to paired non-cancer tissue. High expression levels were associated with poor prognosis in terms of tumour undifferentiation (for LINC-ROR, SOX2, and NANOG), lymph node infiltration (for SOX2), postoperative recurrence (for LINC-ROR and SOX2), and shorter overall survival (OS) and progression-free survival (for all genes studied). The best curve for OS prediction was constructed with LINC-ROR data (area under the receiver operating characteristic curve (AUC) = 0.804 at a cut-off value of 72.7, sensitivity 78.9%, and specificity 80.5%). CONCLUSIONS: Collectively, aberrant LINC-ROR and pluripotent gene expression may be recognised as prognostic markers for RCC. Future functional studies are highly recommended to validate the study findings. Termedia Publishing House 2019-05-17 /pmc/articles/PMC8314397/ /pubmed/34336029 http://dx.doi.org/10.5114/aoms.2019.85201 Text en Copyright: © 2019 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Basic Research
Fawzy, Manal S.
Toraih, Eman A.
El-Wazir, Aya
Hosny, Marwa M.
Badran, Dahlia I.
El Kelish, Amr
Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title_full Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title_fullStr Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title_full_unstemmed Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title_short Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma
title_sort long intergenic non-coding rna, regulator of reprogramming (linc-ror) over-expression predicts poor prognosis in renal cell carcinoma
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314397/
https://www.ncbi.nlm.nih.gov/pubmed/34336029
http://dx.doi.org/10.5114/aoms.2019.85201
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