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Quantification of long non-coding RNAs using qRT-PCR: comparison of different cDNA synthesis methods and RNA stability
INTRODUCTION: Long non-coding RNAs (lncRNAs), a class of regulatory RNA molecules, are over 200 nucleotides long and could be used as a new potential biomarker, but their detection methods such as qRT-PCR are still not validated, and the influence of RNA degradation on lncRNA quantification is not c...
Autores principales: | Kolenda, Tomasz, Ryś, Marcel, Guglas, Kacper, Teresiak, Anna, Bliźniak, Renata, Mackiewicz, Jacek, Lamperska, Katarzyna |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314425/ https://www.ncbi.nlm.nih.gov/pubmed/34336028 http://dx.doi.org/10.5114/aoms.2019.82639 |
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