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Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency

OBJECTIVE: The aim of this study was to assess the safety of poly-lactic co-glycolic acid (PLGA) electrospun membranes as carriers for limbal tissue explants for treatment of limbal stem cell deficiency (LSCD). METHODS AND ANALYSIS: Approval was obtained for a first in-man study from the Drug Contro...

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Autores principales: Ramachandran, Charanya, Deshpande, Pallavi, Ortega, Ilida, Sefat, Farshid, McKean, Rob, Srivastava, Mala, MacNeil, Sheila, Basu, Sayan, Sangwan, Virender Singh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314696/
https://www.ncbi.nlm.nih.gov/pubmed/34395914
http://dx.doi.org/10.1136/bmjophth-2021-000762
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author Ramachandran, Charanya
Deshpande, Pallavi
Ortega, Ilida
Sefat, Farshid
McKean, Rob
Srivastava, Mala
MacNeil, Sheila
Basu, Sayan
Sangwan, Virender Singh
author_facet Ramachandran, Charanya
Deshpande, Pallavi
Ortega, Ilida
Sefat, Farshid
McKean, Rob
Srivastava, Mala
MacNeil, Sheila
Basu, Sayan
Sangwan, Virender Singh
author_sort Ramachandran, Charanya
collection PubMed
description OBJECTIVE: The aim of this study was to assess the safety of poly-lactic co-glycolic acid (PLGA) electrospun membranes as carriers for limbal tissue explants for treatment of limbal stem cell deficiency (LSCD). METHODS AND ANALYSIS: Approval was obtained for a first in-man study from the Drug Controller General of India. PLGA membranes were applied to the affected eye of five patients after removal of the vascular pannus. Simple limbal epithelial transplantation was performed and limbal explants were secured on the membrane using fibrin glue followed by a bandage contact lens. Patients were followed up for 1 year with ocular exams including slit lamp exam, corneal thickness measurements, intraocular pressure measurements and recording of corneal vascularisation and visual acuity. Systemic examinations included pain grading, clinical laboratory assessment, blood chemistry and urine analysis at baseline, 3 and 6 months after surgery. RESULTS: PLGA membranes completely degraded by 8 weeks post-transplantation without any infection or inflammation. In all five patients, the epithelium regenerated by 3 months. In two in five patients, there was a sustained two-line improvement in vision. In one in five patients, the vision improvement was limited due to an underlying stromal scarring. There was recurrence of pannus and LSCD in two in five patients 6 months after surgery which was not attributable to the membrane. The ocular surface remained clear with no epithelial defects in three in five subjects at 12 months. CONCLUSION: PLGA electrospun membranes show promise as carrier for limbal epithelial cells in the treatment of LSCD.
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spelling pubmed-83146962021-08-13 Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency Ramachandran, Charanya Deshpande, Pallavi Ortega, Ilida Sefat, Farshid McKean, Rob Srivastava, Mala MacNeil, Sheila Basu, Sayan Sangwan, Virender Singh BMJ Open Ophthalmol Cornea and Ocular Surface OBJECTIVE: The aim of this study was to assess the safety of poly-lactic co-glycolic acid (PLGA) electrospun membranes as carriers for limbal tissue explants for treatment of limbal stem cell deficiency (LSCD). METHODS AND ANALYSIS: Approval was obtained for a first in-man study from the Drug Controller General of India. PLGA membranes were applied to the affected eye of five patients after removal of the vascular pannus. Simple limbal epithelial transplantation was performed and limbal explants were secured on the membrane using fibrin glue followed by a bandage contact lens. Patients were followed up for 1 year with ocular exams including slit lamp exam, corneal thickness measurements, intraocular pressure measurements and recording of corneal vascularisation and visual acuity. Systemic examinations included pain grading, clinical laboratory assessment, blood chemistry and urine analysis at baseline, 3 and 6 months after surgery. RESULTS: PLGA membranes completely degraded by 8 weeks post-transplantation without any infection or inflammation. In all five patients, the epithelium regenerated by 3 months. In two in five patients, there was a sustained two-line improvement in vision. In one in five patients, the vision improvement was limited due to an underlying stromal scarring. There was recurrence of pannus and LSCD in two in five patients 6 months after surgery which was not attributable to the membrane. The ocular surface remained clear with no epithelial defects in three in five subjects at 12 months. CONCLUSION: PLGA electrospun membranes show promise as carrier for limbal epithelial cells in the treatment of LSCD. BMJ Publishing Group 2021-07-26 /pmc/articles/PMC8314696/ /pubmed/34395914 http://dx.doi.org/10.1136/bmjophth-2021-000762 Text en © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Cornea and Ocular Surface
Ramachandran, Charanya
Deshpande, Pallavi
Ortega, Ilida
Sefat, Farshid
McKean, Rob
Srivastava, Mala
MacNeil, Sheila
Basu, Sayan
Sangwan, Virender Singh
Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title_full Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title_fullStr Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title_full_unstemmed Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title_short Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency
title_sort proof-of-concept study of electrospun plga membrane in the treatment of limbal stem cell deficiency
topic Cornea and Ocular Surface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314696/
https://www.ncbi.nlm.nih.gov/pubmed/34395914
http://dx.doi.org/10.1136/bmjophth-2021-000762
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