Cargando…
The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses
The use of Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae), an economical insect model, for the study of enteropathogenic Escherichia coli (Migula) (EPEC), a diarrheagenic human pathogen, has been demonstrated previously but remains poorly understood. The present study characterizes the Gall...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315237/ https://www.ncbi.nlm.nih.gov/pubmed/34314494 http://dx.doi.org/10.1093/jisesa/ieab046 |
_version_ | 1783729686798401536 |
---|---|
author | Chen, Robin Y Keddie, B Andrew |
author_facet | Chen, Robin Y Keddie, B Andrew |
author_sort | Chen, Robin Y |
collection | PubMed |
description | The use of Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae), an economical insect model, for the study of enteropathogenic Escherichia coli (Migula) (EPEC), a diarrheagenic human pathogen, has been demonstrated previously but remains poorly understood. The present study characterizes the Galleria-EPEC system extensively for future studies using this system. We found that EPEC causes disease in G. mellonella larvae when injected intrahemocoelically but not orally. Disease manifests as increased mortality, decreased survival time, delayed pupation, decreased pupal mass, increased pupal duration, and hemocytopenia. Disease symptoms are dose-dependent and can be used as metrics for measuring EPEC virulence in future studies. The type III secretion system was only partially responsible for EPEC virulence in G. mellonella while the majority of the virulence remains unknown in origin. EPEC elicits insect anti-bacterial immune responses including melanization, hemolymph coagulation, nodulation, and phagocytosis. The immune responses were unable to control EPEC replication in the early stage of infection (≤3 h post-injection). EPEC clearance from the hemocoel does not guarantee insect survival. Overall, this study provided insights into EPEC virulence and pathogenesis in G. mellonella and identified areas of future research using this system. |
format | Online Article Text |
id | pubmed-8315237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83152372021-07-28 The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses Chen, Robin Y Keddie, B Andrew J Insect Sci Research Articles The use of Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae), an economical insect model, for the study of enteropathogenic Escherichia coli (Migula) (EPEC), a diarrheagenic human pathogen, has been demonstrated previously but remains poorly understood. The present study characterizes the Galleria-EPEC system extensively for future studies using this system. We found that EPEC causes disease in G. mellonella larvae when injected intrahemocoelically but not orally. Disease manifests as increased mortality, decreased survival time, delayed pupation, decreased pupal mass, increased pupal duration, and hemocytopenia. Disease symptoms are dose-dependent and can be used as metrics for measuring EPEC virulence in future studies. The type III secretion system was only partially responsible for EPEC virulence in G. mellonella while the majority of the virulence remains unknown in origin. EPEC elicits insect anti-bacterial immune responses including melanization, hemolymph coagulation, nodulation, and phagocytosis. The immune responses were unable to control EPEC replication in the early stage of infection (≤3 h post-injection). EPEC clearance from the hemocoel does not guarantee insect survival. Overall, this study provided insights into EPEC virulence and pathogenesis in G. mellonella and identified areas of future research using this system. Oxford University Press 2021-07-27 /pmc/articles/PMC8315237/ /pubmed/34314494 http://dx.doi.org/10.1093/jisesa/ieab046 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Articles Chen, Robin Y Keddie, B Andrew The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title | The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title_full | The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title_fullStr | The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title_full_unstemmed | The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title_short | The Galleria mellonella-Enteropathogenic Escherichia coli Model System: Characterization of Pathogen Virulence and Insect Immune Responses |
title_sort | galleria mellonella-enteropathogenic escherichia coli model system: characterization of pathogen virulence and insect immune responses |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315237/ https://www.ncbi.nlm.nih.gov/pubmed/34314494 http://dx.doi.org/10.1093/jisesa/ieab046 |
work_keys_str_mv | AT chenrobiny thegalleriamellonellaenteropathogenicescherichiacolimodelsystemcharacterizationofpathogenvirulenceandinsectimmuneresponses AT keddiebandrew thegalleriamellonellaenteropathogenicescherichiacolimodelsystemcharacterizationofpathogenvirulenceandinsectimmuneresponses AT chenrobiny galleriamellonellaenteropathogenicescherichiacolimodelsystemcharacterizationofpathogenvirulenceandinsectimmuneresponses AT keddiebandrew galleriamellonellaenteropathogenicescherichiacolimodelsystemcharacterizationofpathogenvirulenceandinsectimmuneresponses |