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Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse
Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxf...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315533/ https://www.ncbi.nlm.nih.gov/pubmed/34314423 http://dx.doi.org/10.1371/journal.pone.0254138 |
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author | Spradling, Theresa A. Place, Alexandra C. Campbell, Ashley L. Demastes, James W. |
author_facet | Spradling, Theresa A. Place, Alexandra C. Campbell, Ashley L. Demastes, James W. |
author_sort | Spradling, Theresa A. |
collection | PubMed |
description | Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxford Nanopore sequencing coupled with PCR, cloning, and Sanger sequencing to verify structure and arrangement for each chromosome. The genome consisted of 12 circular mitochondrial chromosomes ranging in size from 1,318 to 2,088 nucleotides (nt). Total genome size was 19,015 nt. All 37 genes typical of metazoans (2 rRNA genes, 22 tRNA genes, and 13 protein-coding genes) were present. An average of 26% of each chromosome was composed of non-gene sequences. Within the non-gene region of each chromosome, there was a 79-nt nucleotide sequence that was identical among chromosomes and a conserved sequence with secondary structure that was always followed by a poly-T region. We hypothesize that these regions may be important in the initiation of transcription and DNA replication, respectively. The G. aurei genome shares 8 derived gene clusters with other chewing lice of mammals, but in G. aurei, genes on several chromosomes are not contiguous. |
format | Online Article Text |
id | pubmed-8315533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83155332021-07-31 Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse Spradling, Theresa A. Place, Alexandra C. Campbell, Ashley L. Demastes, James W. PLoS One Research Article Parasitic lice demonstrate an unusual array of mitochondrial genome architectures and gene arrangements. We characterized the mitochondrial genome of Geomydoecus aurei, a chewing louse (Phthiraptera: Trichodectidae) found on pocket gophers (Rodentia: Geomyidae) using reads from both Illumina and Oxford Nanopore sequencing coupled with PCR, cloning, and Sanger sequencing to verify structure and arrangement for each chromosome. The genome consisted of 12 circular mitochondrial chromosomes ranging in size from 1,318 to 2,088 nucleotides (nt). Total genome size was 19,015 nt. All 37 genes typical of metazoans (2 rRNA genes, 22 tRNA genes, and 13 protein-coding genes) were present. An average of 26% of each chromosome was composed of non-gene sequences. Within the non-gene region of each chromosome, there was a 79-nt nucleotide sequence that was identical among chromosomes and a conserved sequence with secondary structure that was always followed by a poly-T region. We hypothesize that these regions may be important in the initiation of transcription and DNA replication, respectively. The G. aurei genome shares 8 derived gene clusters with other chewing lice of mammals, but in G. aurei, genes on several chromosomes are not contiguous. Public Library of Science 2021-07-27 /pmc/articles/PMC8315533/ /pubmed/34314423 http://dx.doi.org/10.1371/journal.pone.0254138 Text en © 2021 Spradling et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Spradling, Theresa A. Place, Alexandra C. Campbell, Ashley L. Demastes, James W. Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title | Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title_full | Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title_fullStr | Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title_full_unstemmed | Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title_short | Mitochondrial genome of Geomydoecus aurei, a pocket-gopher louse |
title_sort | mitochondrial genome of geomydoecus aurei, a pocket-gopher louse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315533/ https://www.ncbi.nlm.nih.gov/pubmed/34314423 http://dx.doi.org/10.1371/journal.pone.0254138 |
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