G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p

G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid–liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with i...

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Autores principales: Gao, Jun, Gao, Zhaofeng, Putnam, Andrea A., Byrd, Alicia K., Venus, Sarah L., Marecki, John C., Edwards, Andrea D., Lowe, Haley M., Jankowsky, Eckhard, Raney, Kevin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315639/
https://www.ncbi.nlm.nih.gov/pubmed/34232232
http://dx.doi.org/10.1039/d1cc01479j
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author Gao, Jun
Gao, Zhaofeng
Putnam, Andrea A.
Byrd, Alicia K.
Venus, Sarah L.
Marecki, John C.
Edwards, Andrea D.
Lowe, Haley M.
Jankowsky, Eckhard
Raney, Kevin D.
author_facet Gao, Jun
Gao, Zhaofeng
Putnam, Andrea A.
Byrd, Alicia K.
Venus, Sarah L.
Marecki, John C.
Edwards, Andrea D.
Lowe, Haley M.
Jankowsky, Eckhard
Raney, Kevin D.
author_sort Gao, Jun
collection PubMed
description G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid–liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains.
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spelling pubmed-83156392021-08-03 G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p Gao, Jun Gao, Zhaofeng Putnam, Andrea A. Byrd, Alicia K. Venus, Sarah L. Marecki, John C. Edwards, Andrea D. Lowe, Haley M. Jankowsky, Eckhard Raney, Kevin D. Chem Commun (Camb) Chemistry G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid–liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains. The Royal Society of Chemistry 2021-07-07 /pmc/articles/PMC8315639/ /pubmed/34232232 http://dx.doi.org/10.1039/d1cc01479j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Gao, Jun
Gao, Zhaofeng
Putnam, Andrea A.
Byrd, Alicia K.
Venus, Sarah L.
Marecki, John C.
Edwards, Andrea D.
Lowe, Haley M.
Jankowsky, Eckhard
Raney, Kevin D.
G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title_full G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title_fullStr G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title_full_unstemmed G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title_short G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p
title_sort g-quadruplex dna inhibits unwinding activity but promotes liquid–liquid phase separation by the dead-box helicase ded1p
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315639/
https://www.ncbi.nlm.nih.gov/pubmed/34232232
http://dx.doi.org/10.1039/d1cc01479j
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