CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis

This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of...

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Detalles Bibliográficos
Autores principales: Nadolinskaia, N. I., Zamakhaev, M. V., Shumkov, M. S., Armianinova, D. K., Karpov, D. S., Goncharenko, A. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315726/
https://www.ncbi.nlm.nih.gov/pubmed/34334794
http://dx.doi.org/10.1134/S0003683821040128
Descripción
Sumario:This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use of genes of Mycobacterium tuberculosis adenylate cyclases. The results revealed the limitations of the pRH2502/pRH2521 system for CRISPR interference associated with the probability of the detection of a protospacer adjacent motif (PAM) in the gene promoter region.

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