CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis

This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of...

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Autores principales: Nadolinskaia, N. I., Zamakhaev, M. V., Shumkov, M. S., Armianinova, D. K., Karpov, D. S., Goncharenko, A. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315726/
https://www.ncbi.nlm.nih.gov/pubmed/34334794
http://dx.doi.org/10.1134/S0003683821040128
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author Nadolinskaia, N. I.
Zamakhaev, M. V.
Shumkov, M. S.
Armianinova, D. K.
Karpov, D. S.
Goncharenko, A. V.
author_facet Nadolinskaia, N. I.
Zamakhaev, M. V.
Shumkov, M. S.
Armianinova, D. K.
Karpov, D. S.
Goncharenko, A. V.
author_sort Nadolinskaia, N. I.
collection PubMed
description This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use of genes of Mycobacterium tuberculosis adenylate cyclases. The results revealed the limitations of the pRH2502/pRH2521 system for CRISPR interference associated with the probability of the detection of a protospacer adjacent motif (PAM) in the gene promoter region.
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spelling pubmed-83157262021-07-28 CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis Nadolinskaia, N. I. Zamakhaev, M. V. Shumkov, M. S. Armianinova, D. K. Karpov, D. S. Goncharenko, A. V. Appl Biochem Microbiol Article This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use of genes of Mycobacterium tuberculosis adenylate cyclases. The results revealed the limitations of the pRH2502/pRH2521 system for CRISPR interference associated with the probability of the detection of a protospacer adjacent motif (PAM) in the gene promoter region. Pleiades Publishing 2021-07-27 2021 /pmc/articles/PMC8315726/ /pubmed/34334794 http://dx.doi.org/10.1134/S0003683821040128 Text en © Pleiades Publishing, Inc. 2021, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 4, pp. 421–425. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 4, pp. 326–331. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Nadolinskaia, N. I.
Zamakhaev, M. V.
Shumkov, M. S.
Armianinova, D. K.
Karpov, D. S.
Goncharenko, A. V.
CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title_full CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title_fullStr CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title_full_unstemmed CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title_short CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
title_sort crispr interference of adenylate cyclases from mycobacterium tuberculosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315726/
https://www.ncbi.nlm.nih.gov/pubmed/34334794
http://dx.doi.org/10.1134/S0003683821040128
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