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CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis

This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of...

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Detalles Bibliográficos
Autores principales:	Nadolinskaia, N. I., Zamakhaev, M. V., Shumkov, M. S., Armianinova, D. K., Karpov, D. S., Goncharenko, A. V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Pleiades Publishing 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315726/ https://www.ncbi.nlm.nih.gov/pubmed/34334794 http://dx.doi.org/10.1134/S0003683821040128

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