Cargando…

Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice

H9N2 avian influenza virus has been continuously circulating among poultry and can infect mammals, indicating that this virus is a potential pandemic strain. During influenza pandemics, secondary bacterial (particularly pneumococcal) pneumonia usually contributes to excessive mortality. In the prese...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jingyun, Wang, Hongyan, Lian, Pengjing, Bai, Yu, Zhang, Zihui, Zhao, Lihong, Xu, Tong, Qiao, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316151/
https://www.ncbi.nlm.nih.gov/pubmed/33722928
http://dx.doi.org/10.1128/IAI.00062-21
_version_ 1783729816545001472
author Li, Jingyun
Wang, Hongyan
Lian, Pengjing
Bai, Yu
Zhang, Zihui
Zhao, Lihong
Xu, Tong
Qiao, Jian
author_facet Li, Jingyun
Wang, Hongyan
Lian, Pengjing
Bai, Yu
Zhang, Zihui
Zhao, Lihong
Xu, Tong
Qiao, Jian
author_sort Li, Jingyun
collection PubMed
description H9N2 avian influenza virus has been continuously circulating among poultry and can infect mammals, indicating that this virus is a potential pandemic strain. During influenza pandemics, secondary bacterial (particularly pneumococcal) pneumonia usually contributes to excessive mortality. In the present study, we observed the dynamic effect of H9N2 virus infection on host defense against secondary pneumococcal infection in mice. BALB/c mice were intranasally inoculated with 1.2 × 10(5) PFU of H9N2 virus followed by 1 × 10(6) CFU of Streptococcus pneumoniae at 7, 14, or 28 days post-H9N2 infection (dpi). The bacterial load, histopathology, body weight, and survival were assessed after pneumococcal infection. Our results showed that H9N2 virus infection had no significant impact on host resistance to secondary pneumococcal infection at 7 dpi. However, H9N2 virus infection increased pulmonary pneumococcal clearance and reduced pneumococcal pneumonia-induced morbidity after secondary pneumococcal infection at 14 or 28 dpi, as reflected by significantly decreased bacterial loads, markedly alleviated pulmonary histopathological changes, and significantly reduced weight loss in mice infected with H9N2 virus followed by S. pneumoniae compared with mice infected only with S. pneumoniae. Further, the significantly decreased bacterial loads were observed when mice were previously infected with a high dose (1.2 × 10(6) PFU) of H9N2 virus. Also, similar to the results obtained in BALB/c mice, improvement in pulmonary pneumococcal clearance was observed in C57BL/6 mice. Overall, our results showed that pulmonary pneumococcal clearance is improved after resolution of H9N2 virus infection in mice.
format Online
Article
Text
id pubmed-8316151
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-83161512021-11-17 Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice Li, Jingyun Wang, Hongyan Lian, Pengjing Bai, Yu Zhang, Zihui Zhao, Lihong Xu, Tong Qiao, Jian Infect Immun Bacterial Infections H9N2 avian influenza virus has been continuously circulating among poultry and can infect mammals, indicating that this virus is a potential pandemic strain. During influenza pandemics, secondary bacterial (particularly pneumococcal) pneumonia usually contributes to excessive mortality. In the present study, we observed the dynamic effect of H9N2 virus infection on host defense against secondary pneumococcal infection in mice. BALB/c mice were intranasally inoculated with 1.2 × 10(5) PFU of H9N2 virus followed by 1 × 10(6) CFU of Streptococcus pneumoniae at 7, 14, or 28 days post-H9N2 infection (dpi). The bacterial load, histopathology, body weight, and survival were assessed after pneumococcal infection. Our results showed that H9N2 virus infection had no significant impact on host resistance to secondary pneumococcal infection at 7 dpi. However, H9N2 virus infection increased pulmonary pneumococcal clearance and reduced pneumococcal pneumonia-induced morbidity after secondary pneumococcal infection at 14 or 28 dpi, as reflected by significantly decreased bacterial loads, markedly alleviated pulmonary histopathological changes, and significantly reduced weight loss in mice infected with H9N2 virus followed by S. pneumoniae compared with mice infected only with S. pneumoniae. Further, the significantly decreased bacterial loads were observed when mice were previously infected with a high dose (1.2 × 10(6) PFU) of H9N2 virus. Also, similar to the results obtained in BALB/c mice, improvement in pulmonary pneumococcal clearance was observed in C57BL/6 mice. Overall, our results showed that pulmonary pneumococcal clearance is improved after resolution of H9N2 virus infection in mice. American Society for Microbiology 2021-05-17 /pmc/articles/PMC8316151/ /pubmed/33722928 http://dx.doi.org/10.1128/IAI.00062-21 Text en Copyright © 2021 Li et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bacterial Infections
Li, Jingyun
Wang, Hongyan
Lian, Pengjing
Bai, Yu
Zhang, Zihui
Zhao, Lihong
Xu, Tong
Qiao, Jian
Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title_full Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title_fullStr Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title_full_unstemmed Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title_short Increased Pulmonary Pneumococcal Clearance after Resolution of H9N2 Avian Influenza Virus Infection in Mice
title_sort increased pulmonary pneumococcal clearance after resolution of h9n2 avian influenza virus infection in mice
topic Bacterial Infections
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316151/
https://www.ncbi.nlm.nih.gov/pubmed/33722928
http://dx.doi.org/10.1128/IAI.00062-21
work_keys_str_mv AT lijingyun increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT wanghongyan increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT lianpengjing increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT baiyu increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT zhangzihui increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT zhaolihong increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT xutong increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice
AT qiaojian increasedpulmonarypneumococcalclearanceafterresolutionofh9n2avianinfluenzavirusinfectioninmice