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Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India

Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the So...

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Autores principales: Annamanedi, Madhavi, Sheela, P., Sundareshan, Srinivasaiah, Isloor, Shrikrishna, Gupta, Priya, Jasmeen, Pagala, Gargi, Madhuranjana, Mallick, Sathi, Hegde, Nagendra R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316343/
https://www.ncbi.nlm.nih.gov/pubmed/34315981
http://dx.doi.org/10.1038/s41598-021-94760-x
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author Annamanedi, Madhavi
Sheela, P.
Sundareshan, Srinivasaiah
Isloor, Shrikrishna
Gupta, Priya
Jasmeen, Pagala
Gargi, Madhuranjana
Mallick, Sathi
Hegde, Nagendra R.
author_facet Annamanedi, Madhavi
Sheela, P.
Sundareshan, Srinivasaiah
Isloor, Shrikrishna
Gupta, Priya
Jasmeen, Pagala
Gargi, Madhuranjana
Mallick, Sathi
Hegde, Nagendra R.
author_sort Annamanedi, Madhavi
collection PubMed
description Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC(+), 44 (26.5%) isolates were mecA(+), with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA(+) but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates.
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spelling pubmed-83163432021-07-28 Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India Annamanedi, Madhavi Sheela, P. Sundareshan, Srinivasaiah Isloor, Shrikrishna Gupta, Priya Jasmeen, Pagala Gargi, Madhuranjana Mallick, Sathi Hegde, Nagendra R. Sci Rep Article Staphylococcus aureus is a major etiological agent of clinical and subclinical bovine mastitis. Owing to the mostly backyard dairy practices, we hypothesized that genetic diversity among mastitis-associated S. aureus from India would be high, and investigated 166 isolates obtained mostly from the Southern State of Karnataka, but also from a few other states. The results revealed (a) 8 to 13 fragments in pulsed-field gel electrophoresis (PFGE), forming 31 distinct patterns, and (b) 34 spa types, of which three (t17680, t18314, and t18320) were newly identified. Multi-locus sequencing typing (MLST) identified 39 sequence types (STs), with ST2454 (34.4%) and ST2459 (24%) being the most commonly represented, which clustered to clonal complexes (CC) CC9 and CC97, respectively; 12 STs were newly identified. Thirty-four (20.5%) of the 166 isolates displayed oxacillin resistance. On the other hand, whereas none were mecC(+), 44 (26.5%) isolates were mecA(+), with a predominance of SCCmecIVb (26/32 isolates, others being untypeable); 24 isolates (14.46%) were oxacillin-susceptible methicillin-resistant S. aureus (OS-MRSA; mecA(+) but OS). Integrated analysis revealed that CC9-ST2454- and CC97-ST2459-SCCmecIVb were the predominant MRSA, although the distribution of CC9 and CC97 was similar between methicillin-resistant and -susceptible isolates. By PCR, 56.25%, 28.75% and 47.5% of the 166 isolates were positive for hlg, tsst and pvl genes, respectively. Our results, for the first time describe the application of a combination of various molecular methods to bovine mastitis-associated S. aureus isolates from India, corroborate the worldwide distribution of CC97 and CC9, and suggest pathogenic potential of the isolates. Nature Publishing Group UK 2021-07-27 /pmc/articles/PMC8316343/ /pubmed/34315981 http://dx.doi.org/10.1038/s41598-021-94760-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Annamanedi, Madhavi
Sheela, P.
Sundareshan, Srinivasaiah
Isloor, Shrikrishna
Gupta, Priya
Jasmeen, Pagala
Gargi, Madhuranjana
Mallick, Sathi
Hegde, Nagendra R.
Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_full Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_fullStr Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_full_unstemmed Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_short Molecular fingerprinting of bovine mastitis-associated Staphylococcus aureus isolates from India
title_sort molecular fingerprinting of bovine mastitis-associated staphylococcus aureus isolates from india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316343/
https://www.ncbi.nlm.nih.gov/pubmed/34315981
http://dx.doi.org/10.1038/s41598-021-94760-x
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