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Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316546/ https://www.ncbi.nlm.nih.gov/pubmed/34315957 http://dx.doi.org/10.1038/s41598-021-94624-4 |
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author | Menezes, Maria Clara Saad Veiga, Alicia Dudy Müller Martins de Lima, Thais Kunimi Kubo Ariga, Suely Vieira Barbeiro, Hermes de Lucena Moreira, Claudia Pinto, Agnes Araujo Sardinha Brandao, Rodrigo Antonio Marchini, Julio Flavio Alencar, Julio Cesar Marino, Lucas Oliveira Gomez, Luz Marina Olsen Saraiva Camara, Niels Souza, Heraldo P. |
author_facet | Menezes, Maria Clara Saad Veiga, Alicia Dudy Müller Martins de Lima, Thais Kunimi Kubo Ariga, Suely Vieira Barbeiro, Hermes de Lucena Moreira, Claudia Pinto, Agnes Araujo Sardinha Brandao, Rodrigo Antonio Marchini, Julio Flavio Alencar, Julio Cesar Marino, Lucas Oliveira Gomez, Luz Marina Olsen Saraiva Camara, Niels Souza, Heraldo P. |
author_sort | Menezes, Maria Clara Saad |
collection | PubMed |
description | The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4. |
format | Online Article Text |
id | pubmed-8316546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83165462021-07-29 Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients Menezes, Maria Clara Saad Veiga, Alicia Dudy Müller Martins de Lima, Thais Kunimi Kubo Ariga, Suely Vieira Barbeiro, Hermes de Lucena Moreira, Claudia Pinto, Agnes Araujo Sardinha Brandao, Rodrigo Antonio Marchini, Julio Flavio Alencar, Julio Cesar Marino, Lucas Oliveira Gomez, Luz Marina Olsen Saraiva Camara, Niels Souza, Heraldo P. Sci Rep Article The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4. Nature Publishing Group UK 2021-07-27 /pmc/articles/PMC8316546/ /pubmed/34315957 http://dx.doi.org/10.1038/s41598-021-94624-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Menezes, Maria Clara Saad Veiga, Alicia Dudy Müller Martins de Lima, Thais Kunimi Kubo Ariga, Suely Vieira Barbeiro, Hermes de Lucena Moreira, Claudia Pinto, Agnes Araujo Sardinha Brandao, Rodrigo Antonio Marchini, Julio Flavio Alencar, Julio Cesar Marino, Lucas Oliveira Gomez, Luz Marina Olsen Saraiva Camara, Niels Souza, Heraldo P. Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title_full | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title_fullStr | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title_full_unstemmed | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title_short | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
title_sort | lower peripheral blood toll-like receptor 3 expression is associated with an unfavorable outcome in severe covid-19 patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316546/ https://www.ncbi.nlm.nih.gov/pubmed/34315957 http://dx.doi.org/10.1038/s41598-021-94624-4 |
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