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Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients

The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines...

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Autores principales: Menezes, Maria Clara Saad, Veiga, Alicia Dudy Müller, Martins de Lima, Thais, Kunimi Kubo Ariga, Suely, Vieira Barbeiro, Hermes, de Lucena Moreira, Claudia, Pinto, Agnes Araujo Sardinha, Brandao, Rodrigo Antonio, Marchini, Julio Flavio, Alencar, Julio Cesar, Marino, Lucas Oliveira, Gomez, Luz Marina, Olsen Saraiva Camara, Niels, Souza, Heraldo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316546/
https://www.ncbi.nlm.nih.gov/pubmed/34315957
http://dx.doi.org/10.1038/s41598-021-94624-4
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author Menezes, Maria Clara Saad
Veiga, Alicia Dudy Müller
Martins de Lima, Thais
Kunimi Kubo Ariga, Suely
Vieira Barbeiro, Hermes
de Lucena Moreira, Claudia
Pinto, Agnes Araujo Sardinha
Brandao, Rodrigo Antonio
Marchini, Julio Flavio
Alencar, Julio Cesar
Marino, Lucas Oliveira
Gomez, Luz Marina
Olsen Saraiva Camara, Niels
Souza, Heraldo P.
author_facet Menezes, Maria Clara Saad
Veiga, Alicia Dudy Müller
Martins de Lima, Thais
Kunimi Kubo Ariga, Suely
Vieira Barbeiro, Hermes
de Lucena Moreira, Claudia
Pinto, Agnes Araujo Sardinha
Brandao, Rodrigo Antonio
Marchini, Julio Flavio
Alencar, Julio Cesar
Marino, Lucas Oliveira
Gomez, Luz Marina
Olsen Saraiva Camara, Niels
Souza, Heraldo P.
author_sort Menezes, Maria Clara Saad
collection PubMed
description The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4.
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spelling pubmed-83165462021-07-29 Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients Menezes, Maria Clara Saad Veiga, Alicia Dudy Müller Martins de Lima, Thais Kunimi Kubo Ariga, Suely Vieira Barbeiro, Hermes de Lucena Moreira, Claudia Pinto, Agnes Araujo Sardinha Brandao, Rodrigo Antonio Marchini, Julio Flavio Alencar, Julio Cesar Marino, Lucas Oliveira Gomez, Luz Marina Olsen Saraiva Camara, Niels Souza, Heraldo P. Sci Rep Article The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4. Nature Publishing Group UK 2021-07-27 /pmc/articles/PMC8316546/ /pubmed/34315957 http://dx.doi.org/10.1038/s41598-021-94624-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Menezes, Maria Clara Saad
Veiga, Alicia Dudy Müller
Martins de Lima, Thais
Kunimi Kubo Ariga, Suely
Vieira Barbeiro, Hermes
de Lucena Moreira, Claudia
Pinto, Agnes Araujo Sardinha
Brandao, Rodrigo Antonio
Marchini, Julio Flavio
Alencar, Julio Cesar
Marino, Lucas Oliveira
Gomez, Luz Marina
Olsen Saraiva Camara, Niels
Souza, Heraldo P.
Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title_full Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title_fullStr Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title_full_unstemmed Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title_short Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
title_sort lower peripheral blood toll-like receptor 3 expression is associated with an unfavorable outcome in severe covid-19 patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316546/
https://www.ncbi.nlm.nih.gov/pubmed/34315957
http://dx.doi.org/10.1038/s41598-021-94624-4
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