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Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice

The mammalian target of rapamycin (mTOR) is an important protein kinase that senses changes in extracellular and intracellular energy levels and plays a key role in regulating energy metabolism. Brown adipose tissue, which can be converted to white adipose tissue, contains a large number of mitochon...

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Autores principales: Huwatibieke, Bahetiyaer, Yin, Wenzhen, Liu, Lingchao, Jin, Yuxin, Xiang, Xinxin, Han, Jingyan, Zhang, Weizhen, Li, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317026/
https://www.ncbi.nlm.nih.gov/pubmed/34335285
http://dx.doi.org/10.3389/fphys.2021.638352
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author Huwatibieke, Bahetiyaer
Yin, Wenzhen
Liu, Lingchao
Jin, Yuxin
Xiang, Xinxin
Han, Jingyan
Zhang, Weizhen
Li, Yin
author_facet Huwatibieke, Bahetiyaer
Yin, Wenzhen
Liu, Lingchao
Jin, Yuxin
Xiang, Xinxin
Han, Jingyan
Zhang, Weizhen
Li, Yin
author_sort Huwatibieke, Bahetiyaer
collection PubMed
description The mammalian target of rapamycin (mTOR) is an important protein kinase that senses changes in extracellular and intracellular energy levels and plays a key role in regulating energy metabolism. Brown adipose tissue, which can be converted to white adipose tissue, contains a large number of mitochondria and regulates energy expenditure through thermogenesis. Because obesity is a process of fat accumulation due to chronic excessive energy intake, we attempted to determine whether the mTOR signaling pathway can affect the mitochondrial quality control of brown adipocytes through sensing energy status, thereby regulating brown/white adipocyte transformation. In the present study, through activation or inhibition of mTOR signaling, we detected mitochondrial biogenesis, dynamics, and autophagy-related markers in brown adipocytes. We found that activation of mTOR signaling downregulated the expression of mitochondrial biogenesis, dynamics, and autophagy-relevant markers and inhibited the mitochondrial quality control of brown adipocytes, indicating a phenotypic transformation of brown to white adipocytes. In contrast, inhibition of mTOR signaling upregulated the expression of mitochondrial biogenesis, dynamics, and mitophagy-relevant markers and strengthened mitochondrial quality control, suggesting an inhibition of the phenotypic transformation of brown to white adipocytes. In conclusion, the mTOR signaling pathway plays an important role in modulating the transformation of adipocytes by regulating mitochondrial quality control.
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spelling pubmed-83170262021-07-29 Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice Huwatibieke, Bahetiyaer Yin, Wenzhen Liu, Lingchao Jin, Yuxin Xiang, Xinxin Han, Jingyan Zhang, Weizhen Li, Yin Front Physiol Physiology The mammalian target of rapamycin (mTOR) is an important protein kinase that senses changes in extracellular and intracellular energy levels and plays a key role in regulating energy metabolism. Brown adipose tissue, which can be converted to white adipose tissue, contains a large number of mitochondria and regulates energy expenditure through thermogenesis. Because obesity is a process of fat accumulation due to chronic excessive energy intake, we attempted to determine whether the mTOR signaling pathway can affect the mitochondrial quality control of brown adipocytes through sensing energy status, thereby regulating brown/white adipocyte transformation. In the present study, through activation or inhibition of mTOR signaling, we detected mitochondrial biogenesis, dynamics, and autophagy-related markers in brown adipocytes. We found that activation of mTOR signaling downregulated the expression of mitochondrial biogenesis, dynamics, and autophagy-relevant markers and inhibited the mitochondrial quality control of brown adipocytes, indicating a phenotypic transformation of brown to white adipocytes. In contrast, inhibition of mTOR signaling upregulated the expression of mitochondrial biogenesis, dynamics, and mitophagy-relevant markers and strengthened mitochondrial quality control, suggesting an inhibition of the phenotypic transformation of brown to white adipocytes. In conclusion, the mTOR signaling pathway plays an important role in modulating the transformation of adipocytes by regulating mitochondrial quality control. Frontiers Media S.A. 2021-07-14 /pmc/articles/PMC8317026/ /pubmed/34335285 http://dx.doi.org/10.3389/fphys.2021.638352 Text en Copyright © 2021 Huwatibieke, Yin, Liu, Jin, Xiang, Han, Zhang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Huwatibieke, Bahetiyaer
Yin, Wenzhen
Liu, Lingchao
Jin, Yuxin
Xiang, Xinxin
Han, Jingyan
Zhang, Weizhen
Li, Yin
Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title_full Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title_fullStr Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title_full_unstemmed Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title_short Mammalian Target of Rapamycin Signaling Pathway Regulates Mitochondrial Quality Control of Brown Adipocytes in Mice
title_sort mammalian target of rapamycin signaling pathway regulates mitochondrial quality control of brown adipocytes in mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317026/
https://www.ncbi.nlm.nih.gov/pubmed/34335285
http://dx.doi.org/10.3389/fphys.2021.638352
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