Cargando…
Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo
Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. I...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317167/ https://www.ncbi.nlm.nih.gov/pubmed/34336784 http://dx.doi.org/10.3389/fchem.2021.676928 |
_version_ | 1783730018332966912 |
---|---|
author | Dong, Shixian Feng, Sijia Chen, Yuzhou Chen, Mo Yang, Yimeng Zhang, Jian Li, Huizhu Li, Xiaotong Ji, Liang Yang, Xing Hao, Yuefeng Chen, Jun Wo, Yan |
author_facet | Dong, Shixian Feng, Sijia Chen, Yuzhou Chen, Mo Yang, Yimeng Zhang, Jian Li, Huizhu Li, Xiaotong Ji, Liang Yang, Xing Hao, Yuefeng Chen, Jun Wo, Yan |
author_sort | Dong, Shixian |
collection | PubMed |
description | Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging in vivo, a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration in vivo, electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring in vivo added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind. |
format | Online Article Text |
id | pubmed-8317167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83171672021-07-29 Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo Dong, Shixian Feng, Sijia Chen, Yuzhou Chen, Mo Yang, Yimeng Zhang, Jian Li, Huizhu Li, Xiaotong Ji, Liang Yang, Xing Hao, Yuefeng Chen, Jun Wo, Yan Front Chem Chemistry Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging in vivo, a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration in vivo, electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring in vivo added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind. Frontiers Media S.A. 2021-07-14 /pmc/articles/PMC8317167/ /pubmed/34336784 http://dx.doi.org/10.3389/fchem.2021.676928 Text en Copyright © 2021 Dong, Feng, Chen, Chen, Yang, Zhang, Li, Li, Ji, Yang, Hao, Chen and Wo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Dong, Shixian Feng, Sijia Chen, Yuzhou Chen, Mo Yang, Yimeng Zhang, Jian Li, Huizhu Li, Xiaotong Ji, Liang Yang, Xing Hao, Yuefeng Chen, Jun Wo, Yan Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title | Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title_full | Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title_fullStr | Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title_full_unstemmed | Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title_short | Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo |
title_sort | nerve suture combined with adscs injection under real-time and dynamic nir-ii fluorescence imaging in peripheral nerve regeneration in vivo |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317167/ https://www.ncbi.nlm.nih.gov/pubmed/34336784 http://dx.doi.org/10.3389/fchem.2021.676928 |
work_keys_str_mv | AT dongshixian nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT fengsijia nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT chenyuzhou nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT chenmo nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT yangyimeng nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT zhangjian nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT lihuizhu nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT lixiaotong nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT jiliang nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT yangxing nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT haoyuefeng nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT chenjun nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo AT woyan nervesuturecombinedwithadscsinjectionunderrealtimeanddynamicniriifluorescenceimaginginperipheralnerveregenerationinvivo |