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Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer

Ovarian cancer is one of the leading causes of female cancer death. Emerging evidence suggests that many dietary natural products have anti-tumorigenic activity, including that of asparagus officinalis. The current study aimed to assess the anti-tumorigenic and anti-metastatic effects of asparagus o...

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Autores principales: Xu, Guangxu, Kong, Weimin, Fang, Ziwei, Fan, Yali, Yin, Yajie, Sullivan, Stephanie A., Tran, Arthur-Quan, Clark, Leslie H., Sun, Wenchuan, Hao, Tianran, Zhao, Luyu, Zhou, Chunxiao, Bae-Jump, Victoria L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317209/
https://www.ncbi.nlm.nih.gov/pubmed/34336674
http://dx.doi.org/10.3389/fonc.2021.688461
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author Xu, Guangxu
Kong, Weimin
Fang, Ziwei
Fan, Yali
Yin, Yajie
Sullivan, Stephanie A.
Tran, Arthur-Quan
Clark, Leslie H.
Sun, Wenchuan
Hao, Tianran
Zhao, Luyu
Zhou, Chunxiao
Bae-Jump, Victoria L.
author_facet Xu, Guangxu
Kong, Weimin
Fang, Ziwei
Fan, Yali
Yin, Yajie
Sullivan, Stephanie A.
Tran, Arthur-Quan
Clark, Leslie H.
Sun, Wenchuan
Hao, Tianran
Zhao, Luyu
Zhou, Chunxiao
Bae-Jump, Victoria L.
author_sort Xu, Guangxu
collection PubMed
description Ovarian cancer is one of the leading causes of female cancer death. Emerging evidence suggests that many dietary natural products have anti-tumorigenic activity, including that of asparagus officinalis. The current study aimed to assess the anti-tumorigenic and anti-metastatic effects of asparagus officinalis on serous ovarian cancer cell lines and a transgenic mouse model of high grade serous ovarian cancer. Asparagus officinalis decreased cellular viability, caused cell cycle G1 phase arrest and induced apoptosis in the OVCAR5 and SKOV3 cells. Induction of apoptosis and inhibition of cell proliferation was rescued by the pan-caspase inhibitor, Z-VAD-FMK, implying that its cytotoxic effects were mainly dependent on caspase pathways. Asparagus officinalis increased levels of ROS and decreased mitochondrial membrane potential with corresponding increases in PERK, Bip, Calnexin PDI and ATF4 in both cell lines. Treatment with asparagus officinalis also reduced ability of adhesion and invasion through epithelial–mesenchymal transition and reduction of VEGF expression. The combination of Asparagus officinalis with paclitaxel had synergistic anti-proliferative activity. Furthermore, Asparagus officinalis significantly inhibited tumor growth and reduced serum VEGF in a genetically engineered mouse model of ovarian cancer under obese and lean conditions, accompanied with a decrease in the expression of Ki67, VEGF and phosphorylated S6, and in an increase in phosphorylation of AMPK in the ovarian tumor tissues. Overall, our data provide a pre-clinical rationale for asparagus officinalis in the prevention and treatment of ovarian cancer as a novel natural product.
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spelling pubmed-83172092021-07-29 Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer Xu, Guangxu Kong, Weimin Fang, Ziwei Fan, Yali Yin, Yajie Sullivan, Stephanie A. Tran, Arthur-Quan Clark, Leslie H. Sun, Wenchuan Hao, Tianran Zhao, Luyu Zhou, Chunxiao Bae-Jump, Victoria L. Front Oncol Oncology Ovarian cancer is one of the leading causes of female cancer death. Emerging evidence suggests that many dietary natural products have anti-tumorigenic activity, including that of asparagus officinalis. The current study aimed to assess the anti-tumorigenic and anti-metastatic effects of asparagus officinalis on serous ovarian cancer cell lines and a transgenic mouse model of high grade serous ovarian cancer. Asparagus officinalis decreased cellular viability, caused cell cycle G1 phase arrest and induced apoptosis in the OVCAR5 and SKOV3 cells. Induction of apoptosis and inhibition of cell proliferation was rescued by the pan-caspase inhibitor, Z-VAD-FMK, implying that its cytotoxic effects were mainly dependent on caspase pathways. Asparagus officinalis increased levels of ROS and decreased mitochondrial membrane potential with corresponding increases in PERK, Bip, Calnexin PDI and ATF4 in both cell lines. Treatment with asparagus officinalis also reduced ability of adhesion and invasion through epithelial–mesenchymal transition and reduction of VEGF expression. The combination of Asparagus officinalis with paclitaxel had synergistic anti-proliferative activity. Furthermore, Asparagus officinalis significantly inhibited tumor growth and reduced serum VEGF in a genetically engineered mouse model of ovarian cancer under obese and lean conditions, accompanied with a decrease in the expression of Ki67, VEGF and phosphorylated S6, and in an increase in phosphorylation of AMPK in the ovarian tumor tissues. Overall, our data provide a pre-clinical rationale for asparagus officinalis in the prevention and treatment of ovarian cancer as a novel natural product. Frontiers Media S.A. 2021-07-14 /pmc/articles/PMC8317209/ /pubmed/34336674 http://dx.doi.org/10.3389/fonc.2021.688461 Text en Copyright © 2021 Xu, Kong, Fang, Fan, Yin, Sullivan, Tran, Clark, Sun, Hao, Zhao, Zhou and Bae-Jump https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Xu, Guangxu
Kong, Weimin
Fang, Ziwei
Fan, Yali
Yin, Yajie
Sullivan, Stephanie A.
Tran, Arthur-Quan
Clark, Leslie H.
Sun, Wenchuan
Hao, Tianran
Zhao, Luyu
Zhou, Chunxiao
Bae-Jump, Victoria L.
Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title_full Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title_fullStr Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title_full_unstemmed Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title_short Asparagus officinalis Exhibits Anti-Tumorigenic and Anti-Metastatic Effects in Ovarian Cancer
title_sort asparagus officinalis exhibits anti-tumorigenic and anti-metastatic effects in ovarian cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317209/
https://www.ncbi.nlm.nih.gov/pubmed/34336674
http://dx.doi.org/10.3389/fonc.2021.688461
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