Cargando…

Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis

Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to as...

Descripción completa

Detalles Bibliográficos
Autores principales: Gholizadeh, Pourya, Aghazadeh, Mohammad, Ghotaslou, Reza, Rezaee, Mohammad Ahangarzadeh, Pirzadeh, Tahereh, Cui, Longzhu, Watanabe, Shinya, Feizi, Hadi, Kadkhoda, Hiva, Kafil, Hossein Samadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317297/
https://www.ncbi.nlm.nih.gov/pubmed/34321002
http://dx.doi.org/10.1186/s12941-021-00455-6
_version_ 1783730044087042048
author Gholizadeh, Pourya
Aghazadeh, Mohammad
Ghotaslou, Reza
Rezaee, Mohammad Ahangarzadeh
Pirzadeh, Tahereh
Cui, Longzhu
Watanabe, Shinya
Feizi, Hadi
Kadkhoda, Hiva
Kafil, Hossein Samadi
author_facet Gholizadeh, Pourya
Aghazadeh, Mohammad
Ghotaslou, Reza
Rezaee, Mohammad Ahangarzadeh
Pirzadeh, Tahereh
Cui, Longzhu
Watanabe, Shinya
Feizi, Hadi
Kadkhoda, Hiva
Kafil, Hossein Samadi
author_sort Gholizadeh, Pourya
collection PubMed
description Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to assess correlation of the CRISPR-Cas system distribution with the acquisition of antibiotic resistance among E. faecalis isolates. A total of 151 isolates of E. faecalis were collected from urinary tract infections (UTI) and dental-root canal (DRC). All isolates were screened for phenotypic antibiotic resistance. In addition, antibiotic resistance genes and CRISPR loci were screened by using polymerase chain reaction. Genomic background of the isolates was identified by random amplified polymorphic DNA (RAPD)-PCR. The number of multidrug-resistant E. faecalis strains were higher in UTI isolates than in DRC isolates. RAPD-PCR confirmed that genomic background was diverse in UTI and DRC isolates used in this study. CRISPR loci were highly accumulated in gentamycin-, teicoplanin-, erythromycin-, and tetracycline-susceptible strains. In concordance with drug susceptibility, smaller number of CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE, and ant(6) positive strains. These data indicate a negative correlation between CRISPR-cas loci and antibiotic resistance, as well as, carriage of antibiotic resistant genes in both of UTI and DRC isolates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12941-021-00455-6.
format Online
Article
Text
id pubmed-8317297
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-83172972021-07-28 Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis Gholizadeh, Pourya Aghazadeh, Mohammad Ghotaslou, Reza Rezaee, Mohammad Ahangarzadeh Pirzadeh, Tahereh Cui, Longzhu Watanabe, Shinya Feizi, Hadi Kadkhoda, Hiva Kafil, Hossein Samadi Ann Clin Microbiol Antimicrob Research Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to assess correlation of the CRISPR-Cas system distribution with the acquisition of antibiotic resistance among E. faecalis isolates. A total of 151 isolates of E. faecalis were collected from urinary tract infections (UTI) and dental-root canal (DRC). All isolates were screened for phenotypic antibiotic resistance. In addition, antibiotic resistance genes and CRISPR loci were screened by using polymerase chain reaction. Genomic background of the isolates was identified by random amplified polymorphic DNA (RAPD)-PCR. The number of multidrug-resistant E. faecalis strains were higher in UTI isolates than in DRC isolates. RAPD-PCR confirmed that genomic background was diverse in UTI and DRC isolates used in this study. CRISPR loci were highly accumulated in gentamycin-, teicoplanin-, erythromycin-, and tetracycline-susceptible strains. In concordance with drug susceptibility, smaller number of CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE, and ant(6) positive strains. These data indicate a negative correlation between CRISPR-cas loci and antibiotic resistance, as well as, carriage of antibiotic resistant genes in both of UTI and DRC isolates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12941-021-00455-6. BioMed Central 2021-07-28 /pmc/articles/PMC8317297/ /pubmed/34321002 http://dx.doi.org/10.1186/s12941-021-00455-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Gholizadeh, Pourya
Aghazadeh, Mohammad
Ghotaslou, Reza
Rezaee, Mohammad Ahangarzadeh
Pirzadeh, Tahereh
Cui, Longzhu
Watanabe, Shinya
Feizi, Hadi
Kadkhoda, Hiva
Kafil, Hossein Samadi
Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title_full Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title_fullStr Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title_full_unstemmed Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title_short Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
title_sort role of crispr-cas system on antibiotic resistance patterns of enterococcus faecalis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317297/
https://www.ncbi.nlm.nih.gov/pubmed/34321002
http://dx.doi.org/10.1186/s12941-021-00455-6
work_keys_str_mv AT gholizadehpourya roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT aghazadehmohammad roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT ghotasloureza roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT rezaeemohammadahangarzadeh roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT pirzadehtahereh roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT cuilongzhu roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT watanabeshinya roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT feizihadi roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT kadkhodahiva roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis
AT kafilhosseinsamadi roleofcrisprcassystemonantibioticresistancepatternsofenterococcusfaecalis