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Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to as...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317297/ https://www.ncbi.nlm.nih.gov/pubmed/34321002 http://dx.doi.org/10.1186/s12941-021-00455-6 |
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author | Gholizadeh, Pourya Aghazadeh, Mohammad Ghotaslou, Reza Rezaee, Mohammad Ahangarzadeh Pirzadeh, Tahereh Cui, Longzhu Watanabe, Shinya Feizi, Hadi Kadkhoda, Hiva Kafil, Hossein Samadi |
author_facet | Gholizadeh, Pourya Aghazadeh, Mohammad Ghotaslou, Reza Rezaee, Mohammad Ahangarzadeh Pirzadeh, Tahereh Cui, Longzhu Watanabe, Shinya Feizi, Hadi Kadkhoda, Hiva Kafil, Hossein Samadi |
author_sort | Gholizadeh, Pourya |
collection | PubMed |
description | Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to assess correlation of the CRISPR-Cas system distribution with the acquisition of antibiotic resistance among E. faecalis isolates. A total of 151 isolates of E. faecalis were collected from urinary tract infections (UTI) and dental-root canal (DRC). All isolates were screened for phenotypic antibiotic resistance. In addition, antibiotic resistance genes and CRISPR loci were screened by using polymerase chain reaction. Genomic background of the isolates was identified by random amplified polymorphic DNA (RAPD)-PCR. The number of multidrug-resistant E. faecalis strains were higher in UTI isolates than in DRC isolates. RAPD-PCR confirmed that genomic background was diverse in UTI and DRC isolates used in this study. CRISPR loci were highly accumulated in gentamycin-, teicoplanin-, erythromycin-, and tetracycline-susceptible strains. In concordance with drug susceptibility, smaller number of CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE, and ant(6) positive strains. These data indicate a negative correlation between CRISPR-cas loci and antibiotic resistance, as well as, carriage of antibiotic resistant genes in both of UTI and DRC isolates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12941-021-00455-6. |
format | Online Article Text |
id | pubmed-8317297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83172972021-07-28 Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis Gholizadeh, Pourya Aghazadeh, Mohammad Ghotaslou, Reza Rezaee, Mohammad Ahangarzadeh Pirzadeh, Tahereh Cui, Longzhu Watanabe, Shinya Feizi, Hadi Kadkhoda, Hiva Kafil, Hossein Samadi Ann Clin Microbiol Antimicrob Research Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are one of the factors which can contribute to limiting the development and evolution of antibiotic resistance in bacteria. There are three genomic loci of CRISPR-Cas in Enterococcus faecalis. In this study, we aimed to assess correlation of the CRISPR-Cas system distribution with the acquisition of antibiotic resistance among E. faecalis isolates. A total of 151 isolates of E. faecalis were collected from urinary tract infections (UTI) and dental-root canal (DRC). All isolates were screened for phenotypic antibiotic resistance. In addition, antibiotic resistance genes and CRISPR loci were screened by using polymerase chain reaction. Genomic background of the isolates was identified by random amplified polymorphic DNA (RAPD)-PCR. The number of multidrug-resistant E. faecalis strains were higher in UTI isolates than in DRC isolates. RAPD-PCR confirmed that genomic background was diverse in UTI and DRC isolates used in this study. CRISPR loci were highly accumulated in gentamycin-, teicoplanin-, erythromycin-, and tetracycline-susceptible strains. In concordance with drug susceptibility, smaller number of CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE, and ant(6) positive strains. These data indicate a negative correlation between CRISPR-cas loci and antibiotic resistance, as well as, carriage of antibiotic resistant genes in both of UTI and DRC isolates. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12941-021-00455-6. BioMed Central 2021-07-28 /pmc/articles/PMC8317297/ /pubmed/34321002 http://dx.doi.org/10.1186/s12941-021-00455-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Gholizadeh, Pourya Aghazadeh, Mohammad Ghotaslou, Reza Rezaee, Mohammad Ahangarzadeh Pirzadeh, Tahereh Cui, Longzhu Watanabe, Shinya Feizi, Hadi Kadkhoda, Hiva Kafil, Hossein Samadi Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title | Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title_full | Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title_fullStr | Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title_full_unstemmed | Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title_short | Role of CRISPR-Cas system on antibiotic resistance patterns of Enterococcus faecalis |
title_sort | role of crispr-cas system on antibiotic resistance patterns of enterococcus faecalis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317297/ https://www.ncbi.nlm.nih.gov/pubmed/34321002 http://dx.doi.org/10.1186/s12941-021-00455-6 |
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