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Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function
BACKGROUND: Osteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function. METHODS: One hundred eight pairs of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317387/ https://www.ncbi.nlm.nih.gov/pubmed/34321018 http://dx.doi.org/10.1186/s13018-021-02611-2 |
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author | Mi, Zhanhu Dong, Yanyun Wang, Zhibiao Ye, Peng |
author_facet | Mi, Zhanhu Dong, Yanyun Wang, Zhibiao Ye, Peng |
author_sort | Mi, Zhanhu |
collection | PubMed |
description | BACKGROUND: Osteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function. METHODS: One hundred eight pairs of tissues were collected, and OS cell lines were purchased. lncRNA GNAS-AS1 expression in these tissues and cells were analyzed by qRT-PCR. Clinical data were analyzed using chi-square tests, Kaplan-Meier curves (log-rank test), and Cox regression. CCK-8 and transwell assay were conducted to analyze the effect of lncRNA GNAS-AS1 on cell proliferation, invasion, and migration. The downstream miRNA was presumed. RESULTS: The expression of lncRNA GNAS-AS1 was significantly increased in OS cells and tissues, and related to Enneking staging and distant metastasis. Patients with high lncRNA GNAS-AS1 expression represented shorter overall survival and was an independent prognostic predictor of OS. LncRNA GNAS-AS1 knockdown inhibited cell proliferation, migration, and invasion by regulated miR-490-3p partly at least. CONCLUSIONS: LncRNA GNAS-AS1 can be used as a prognostic indicator and its inhibition suppress the development of OS, suggesting its value as novel therapeutic strategies in OS. |
format | Online Article Text |
id | pubmed-8317387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83173872021-07-30 Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function Mi, Zhanhu Dong, Yanyun Wang, Zhibiao Ye, Peng J Orthop Surg Res Research Article BACKGROUND: Osteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function. METHODS: One hundred eight pairs of tissues were collected, and OS cell lines were purchased. lncRNA GNAS-AS1 expression in these tissues and cells were analyzed by qRT-PCR. Clinical data were analyzed using chi-square tests, Kaplan-Meier curves (log-rank test), and Cox regression. CCK-8 and transwell assay were conducted to analyze the effect of lncRNA GNAS-AS1 on cell proliferation, invasion, and migration. The downstream miRNA was presumed. RESULTS: The expression of lncRNA GNAS-AS1 was significantly increased in OS cells and tissues, and related to Enneking staging and distant metastasis. Patients with high lncRNA GNAS-AS1 expression represented shorter overall survival and was an independent prognostic predictor of OS. LncRNA GNAS-AS1 knockdown inhibited cell proliferation, migration, and invasion by regulated miR-490-3p partly at least. CONCLUSIONS: LncRNA GNAS-AS1 can be used as a prognostic indicator and its inhibition suppress the development of OS, suggesting its value as novel therapeutic strategies in OS. BioMed Central 2021-07-28 /pmc/articles/PMC8317387/ /pubmed/34321018 http://dx.doi.org/10.1186/s13018-021-02611-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Mi, Zhanhu Dong, Yanyun Wang, Zhibiao Ye, Peng Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title | Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title_full | Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title_fullStr | Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title_full_unstemmed | Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title_short | Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function |
title_sort | biomarker potential of lncrna gnas-as1 in osteosarcoma prognosis and effect on cellular function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317387/ https://www.ncbi.nlm.nih.gov/pubmed/34321018 http://dx.doi.org/10.1186/s13018-021-02611-2 |
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