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Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study
To describe how 17β-estradiol (E2) influence in the monocyte/macrophage response induced by S. aureus in in vitro models of murine peritoneal macrophages (MPMs) and human peripheral blood monocytes (HPBM). MPMs (2 x 10(5)/ml) were isolated from sham (n=3) and ovariectomized (OVX) females (n = 3) and...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317603/ https://www.ncbi.nlm.nih.gov/pubmed/34336722 http://dx.doi.org/10.3389/fcimb.2021.701391 |
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author | Souza, Clarissa Leal Silva e Barbosa, Camila Dutra Coelho, Hanna I. L. N. Santos Júnior, Manoel N. Barbosa, Elaine Novaes Queiroz, Éllunny Chaves Teles, Mauro Fernandes dos Santos, Déborah Cruz Bittencourt, Rafaela Souza Soares, Telma de Jesus Oliveira, Márcio Vasconcelos Timenetsky, Jorge Campos, Guilherme Barreto Marques, Lucas Miranda |
author_facet | Souza, Clarissa Leal Silva e Barbosa, Camila Dutra Coelho, Hanna I. L. N. Santos Júnior, Manoel N. Barbosa, Elaine Novaes Queiroz, Éllunny Chaves Teles, Mauro Fernandes dos Santos, Déborah Cruz Bittencourt, Rafaela Souza Soares, Telma de Jesus Oliveira, Márcio Vasconcelos Timenetsky, Jorge Campos, Guilherme Barreto Marques, Lucas Miranda |
author_sort | Souza, Clarissa Leal Silva e |
collection | PubMed |
description | To describe how 17β-estradiol (E2) influence in the monocyte/macrophage response induced by S. aureus in in vitro models of murine peritoneal macrophages (MPMs) and human peripheral blood monocytes (HPBM). MPMs (2 x 10(5)/ml) were isolated from sham (n=3) and ovariectomized (OVX) females (n = 3) and males (n = 3) after induction by thioglycolate. The MPMs obtained from OVX females and males were treated for 24 hours with 17β-estradiol (E2) (10(-7) M), and after that, inoculation with S. aureus was carried out for 6 hours. The macrophages were collected and destined to evaluate the relative gene expression of TNF-α, IL-1β, IL-6, IL-8 and TLR2. For the in vitro model of HPBMs, six men and six women of childbearing age were selected and HPBMs were isolated from samples of the volunteers’ peripheral blood. In women, blood was collected both during menstruation and in the periovulatory period. HPBMs were inoculated with S. aureus for 6 hours and the supernatant was collected for analysis of cytokines by Luminex and the HPBMs were removed for analysis of 84 genes involved in the host’s response to bacterial infections by RT-PCR array. Previous treatment with E2 decreased the gene expression and production of proinflammatory cytokines, such as TNF-α, IL-1β and IL-6 and decreased the expression of TLR2 tanto em MPMs quanto em HPBMs. The analysis of gene expression shows that E2 inhibited the NFκB pathway. It is suggested that 17β-estradiol acts as an immunoprotective in the monocyte/macrophage response induced by S. aureus. |
format | Online Article Text |
id | pubmed-8317603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83176032021-07-29 Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study Souza, Clarissa Leal Silva e Barbosa, Camila Dutra Coelho, Hanna I. L. N. Santos Júnior, Manoel N. Barbosa, Elaine Novaes Queiroz, Éllunny Chaves Teles, Mauro Fernandes dos Santos, Déborah Cruz Bittencourt, Rafaela Souza Soares, Telma de Jesus Oliveira, Márcio Vasconcelos Timenetsky, Jorge Campos, Guilherme Barreto Marques, Lucas Miranda Front Cell Infect Microbiol Cellular and Infection Microbiology To describe how 17β-estradiol (E2) influence in the monocyte/macrophage response induced by S. aureus in in vitro models of murine peritoneal macrophages (MPMs) and human peripheral blood monocytes (HPBM). MPMs (2 x 10(5)/ml) were isolated from sham (n=3) and ovariectomized (OVX) females (n = 3) and males (n = 3) after induction by thioglycolate. The MPMs obtained from OVX females and males were treated for 24 hours with 17β-estradiol (E2) (10(-7) M), and after that, inoculation with S. aureus was carried out for 6 hours. The macrophages were collected and destined to evaluate the relative gene expression of TNF-α, IL-1β, IL-6, IL-8 and TLR2. For the in vitro model of HPBMs, six men and six women of childbearing age were selected and HPBMs were isolated from samples of the volunteers’ peripheral blood. In women, blood was collected both during menstruation and in the periovulatory period. HPBMs were inoculated with S. aureus for 6 hours and the supernatant was collected for analysis of cytokines by Luminex and the HPBMs were removed for analysis of 84 genes involved in the host’s response to bacterial infections by RT-PCR array. Previous treatment with E2 decreased the gene expression and production of proinflammatory cytokines, such as TNF-α, IL-1β and IL-6 and decreased the expression of TLR2 tanto em MPMs quanto em HPBMs. The analysis of gene expression shows that E2 inhibited the NFκB pathway. It is suggested that 17β-estradiol acts as an immunoprotective in the monocyte/macrophage response induced by S. aureus. Frontiers Media S.A. 2021-07-14 /pmc/articles/PMC8317603/ /pubmed/34336722 http://dx.doi.org/10.3389/fcimb.2021.701391 Text en Copyright © 2021 Souza, Barbosa, Coelho, Santos Júnior, Barbosa, Queiroz, Teles, dos Santos, Bittencourt, Soares, Oliveira, Timenetsky, Campos and Marques https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Souza, Clarissa Leal Silva e Barbosa, Camila Dutra Coelho, Hanna I. L. N. Santos Júnior, Manoel N. Barbosa, Elaine Novaes Queiroz, Éllunny Chaves Teles, Mauro Fernandes dos Santos, Déborah Cruz Bittencourt, Rafaela Souza Soares, Telma de Jesus Oliveira, Márcio Vasconcelos Timenetsky, Jorge Campos, Guilherme Barreto Marques, Lucas Miranda Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title | Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title_full | Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title_fullStr | Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title_full_unstemmed | Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title_short | Effects of 17β-Estradiol on Monocyte/Macrophage Response to Staphylococcus aureus: An In Vitro Study |
title_sort | effects of 17β-estradiol on monocyte/macrophage response to staphylococcus aureus: an in vitro study |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317603/ https://www.ncbi.nlm.nih.gov/pubmed/34336722 http://dx.doi.org/10.3389/fcimb.2021.701391 |
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