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Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model
Objective and Impact Statement. This is the first longitudinal study investigating the effects of histotripsy on local tumor progression in an in vivo orthotopic, immunocompetent rat hepatocellular carcinoma (HCC) model. Introduction. Histotripsy is the first noninvasive, nonionizing, nonthermal, me...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318009/ https://www.ncbi.nlm.nih.gov/pubmed/34327513 http://dx.doi.org/10.34133/2020/9830304 |
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author | Worlikar, Tejaswi Mendiratta-Lala, Mishal Vlaisavljevich, Eli Hubbard, Ryan Shi, Jiaqi Hall, Timothy L. Cho, Clifford S. Lee, Fred T. Greve, Joan Xu, Zhen |
author_facet | Worlikar, Tejaswi Mendiratta-Lala, Mishal Vlaisavljevich, Eli Hubbard, Ryan Shi, Jiaqi Hall, Timothy L. Cho, Clifford S. Lee, Fred T. Greve, Joan Xu, Zhen |
author_sort | Worlikar, Tejaswi |
collection | PubMed |
description | Objective and Impact Statement. This is the first longitudinal study investigating the effects of histotripsy on local tumor progression in an in vivo orthotopic, immunocompetent rat hepatocellular carcinoma (HCC) model. Introduction. Histotripsy is the first noninvasive, nonionizing, nonthermal, mechanical ablation technique using ultrasound to generate acoustic cavitation to liquefy the target tissue into acellular debris with millimeter accuracy. Previously, histotripsy has demonstrated in vivo ablation of noncancerous liver tissue. Methods. N1-S1 HCC tumors were generated in the livers of immunocompetent rats ([Formula: see text] , control; [Formula: see text] , treatment). Real-time ultrasound-guided histotripsy was applied to ablate either [Formula: see text] ([Formula: see text] , complete treatment) or 50-75% tumor volume ([Formula: see text] , partial treatment) by delivering 1-2 cycle histotripsy pulses at 100 Hz PRF (pulse repetition frequency) with [Formula: see text] MPa using a custom 1 MHz transducer. Rats were monitored weekly using MRI (magnetic resonance imaging) for 3 months or until tumors reached ~25 mm. Results. MRI revealed effective post-histotripsy reduction of tumor burden with near-complete resorption of the ablated tumor in 14/15 (93.3%) treated rats. Histopathology showed <5 mm shrunken, non-tumoral, fibrous tissue at the treatment site at 3 months. Rats with increased tumor burden (3/6 control and 1 partial treatment) were euthanized early by 2-4 weeks. In 3 other controls, histology revealed fibrous tissue at original tumor site at 3 months. There was no evidence of histotripsy-induced off-target tissue injury. Conclusion. Complete and partial histotripsy ablation resulted in effective tumor removal for 14/15 rats, with no evidence of local tumor progression or recurrence. |
format | Online Article Text |
id | pubmed-8318009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-83180092021-07-28 Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model Worlikar, Tejaswi Mendiratta-Lala, Mishal Vlaisavljevich, Eli Hubbard, Ryan Shi, Jiaqi Hall, Timothy L. Cho, Clifford S. Lee, Fred T. Greve, Joan Xu, Zhen BME Front Research Article Objective and Impact Statement. This is the first longitudinal study investigating the effects of histotripsy on local tumor progression in an in vivo orthotopic, immunocompetent rat hepatocellular carcinoma (HCC) model. Introduction. Histotripsy is the first noninvasive, nonionizing, nonthermal, mechanical ablation technique using ultrasound to generate acoustic cavitation to liquefy the target tissue into acellular debris with millimeter accuracy. Previously, histotripsy has demonstrated in vivo ablation of noncancerous liver tissue. Methods. N1-S1 HCC tumors were generated in the livers of immunocompetent rats ([Formula: see text] , control; [Formula: see text] , treatment). Real-time ultrasound-guided histotripsy was applied to ablate either [Formula: see text] ([Formula: see text] , complete treatment) or 50-75% tumor volume ([Formula: see text] , partial treatment) by delivering 1-2 cycle histotripsy pulses at 100 Hz PRF (pulse repetition frequency) with [Formula: see text] MPa using a custom 1 MHz transducer. Rats were monitored weekly using MRI (magnetic resonance imaging) for 3 months or until tumors reached ~25 mm. Results. MRI revealed effective post-histotripsy reduction of tumor burden with near-complete resorption of the ablated tumor in 14/15 (93.3%) treated rats. Histopathology showed <5 mm shrunken, non-tumoral, fibrous tissue at the treatment site at 3 months. Rats with increased tumor burden (3/6 control and 1 partial treatment) were euthanized early by 2-4 weeks. In 3 other controls, histology revealed fibrous tissue at original tumor site at 3 months. There was no evidence of histotripsy-induced off-target tissue injury. Conclusion. Complete and partial histotripsy ablation resulted in effective tumor removal for 14/15 rats, with no evidence of local tumor progression or recurrence. AAAS 2020-11-25 /pmc/articles/PMC8318009/ /pubmed/34327513 http://dx.doi.org/10.34133/2020/9830304 Text en Copyright © 2020 Tejaswi Worlikar et al. https://creativecommons.org/licenses/by/4.0/Exclusive Licensee Suzhou Institute of Biomedical Engineering and Technology, CAS. Distributed under a Creative Commons Attribution License (CC BY 4.0). (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Research Article Worlikar, Tejaswi Mendiratta-Lala, Mishal Vlaisavljevich, Eli Hubbard, Ryan Shi, Jiaqi Hall, Timothy L. Cho, Clifford S. Lee, Fred T. Greve, Joan Xu, Zhen Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title | Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title_full | Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title_fullStr | Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title_full_unstemmed | Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title_short | Effects of Histotripsy on Local Tumor Progression in an in vivo Orthotopic Rodent Liver Tumor Model |
title_sort | effects of histotripsy on local tumor progression in an in vivo orthotopic rodent liver tumor model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318009/ https://www.ncbi.nlm.nih.gov/pubmed/34327513 http://dx.doi.org/10.34133/2020/9830304 |
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