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Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection

The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. The host-encoded microRNA (miRNA) response to SARS-CoV-2 infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally a...

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Detalles Bibliográficos
Autores principales: Farr, Ryan J., Rootes, Christina L., Rowntree, Louise C., Nguyen, Thi H. O., Hensen, Luca, Kedzierski, Lukasz, Cheng, Allen C., Kedzierska, Katherine, Au, Gough G., Marsh, Glenn A., Vasan, Seshadri S., Foo, Chwan Hong, Cowled, Christopher, Stewart, Cameron R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318295/
https://www.ncbi.nlm.nih.gov/pubmed/34320031
http://dx.doi.org/10.1371/journal.ppat.1009759
Descripción
Sumario:The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. The host-encoded microRNA (miRNA) response to SARS-CoV-2 infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally and ten age and gender matched healthy donors. We observed 55 miRNAs that were altered in COVID-19 patients during early-stage disease, with the inflammatory miR-31-5p the most strongly upregulated. Supervised machine learning analysis revealed that a three-miRNA signature (miR-423-5p, miR-23a-3p and miR-195-5p) independently classified COVID-19 cases with an accuracy of 99.9%. In a ferret COVID-19 model, the three-miRNA signature again detected SARS-CoV-2 infection with 99.7% accuracy, and distinguished SARS-CoV-2 infection from influenza A (H1N1) infection and healthy controls with 95% accuracy. Distinct miRNA profiles were also observed in COVID-19 patients requiring oxygenation. This study demonstrates that SARS-CoV-2 infection induces a robust host miRNA response that could improve COVID-19 detection and patient management.