Identification of a mimotope of an infectious bronchitis virus S1 protein

The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected ph...

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Autores principales: Zhou, Jingming, Li, Jianan, Li, Yanghui, Liu, Hongliang, Qi, Yanhua, Wang, Aiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2021
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318793/
https://www.ncbi.nlm.nih.gov/pubmed/34170090
http://dx.doi.org/10.4142/jvs.2021.22.e49
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author Zhou, Jingming
Li, Jianan
Li, Yanghui
Liu, Hongliang
Qi, Yanhua
Wang, Aiping
author_facet Zhou, Jingming
Li, Jianan
Li, Yanghui
Liu, Hongliang
Qi, Yanhua
Wang, Aiping
author_sort Zhou, Jingming
collection PubMed
description The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzyme-linked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.
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spelling pubmed-83187932021-08-02 Identification of a mimotope of an infectious bronchitis virus S1 protein Zhou, Jingming Li, Jianan Li, Yanghui Liu, Hongliang Qi, Yanhua Wang, Aiping J Vet Sci Rapid Communication The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzyme-linked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein. The Korean Society of Veterinary Science 2021-07 2021-06-02 /pmc/articles/PMC8318793/ /pubmed/34170090 http://dx.doi.org/10.4142/jvs.2021.22.e49 Text en © 2021 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Rapid Communication
Zhou, Jingming
Li, Jianan
Li, Yanghui
Liu, Hongliang
Qi, Yanhua
Wang, Aiping
Identification of a mimotope of an infectious bronchitis virus S1 protein
title Identification of a mimotope of an infectious bronchitis virus S1 protein
title_full Identification of a mimotope of an infectious bronchitis virus S1 protein
title_fullStr Identification of a mimotope of an infectious bronchitis virus S1 protein
title_full_unstemmed Identification of a mimotope of an infectious bronchitis virus S1 protein
title_short Identification of a mimotope of an infectious bronchitis virus S1 protein
title_sort identification of a mimotope of an infectious bronchitis virus s1 protein
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318793/
https://www.ncbi.nlm.nih.gov/pubmed/34170090
http://dx.doi.org/10.4142/jvs.2021.22.e49
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