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Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry
Although nucleos(t)ide analogs and interferons suppress hepatitis B virus (HBV) replication, they must be taken continuously and have a low response rate. Therefore, therapeutics for HBV with novel modes of action are needed. Humanized virus-suppressing factor (hzVSF) is a monoclonal antibody agains...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319007/ https://www.ncbi.nlm.nih.gov/pubmed/34345745 http://dx.doi.org/10.1016/j.heliyon.2021.e07586 |
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author | Miyakawa, Yu Otsuka, Motoyuki Sekiba, Kazuma Funato, Kazuyoshi Koike, Kazuhiko |
author_facet | Miyakawa, Yu Otsuka, Motoyuki Sekiba, Kazuma Funato, Kazuyoshi Koike, Kazuhiko |
author_sort | Miyakawa, Yu |
collection | PubMed |
description | Although nucleos(t)ide analogs and interferons suppress hepatitis B virus (HBV) replication, they must be taken continuously and have a low response rate. Therefore, therapeutics for HBV with novel modes of action are needed. Humanized virus-suppressing factor (hzVSF) is a monoclonal antibody against vimentin that exhibits broad-spectrum antiviral activity. Here, hzVSF significantly inhibited HBV infection. Although hzVSF inhibited HBV RNA production, it did not affect viral transcription from minicircle DNA mimicking covalently closed circular DNA. Additionally, hzVSF did not inhibit viral protein or DNA release from infected cells. Rather, hzVSF inhibited the cell entry of viral preS1 peptides, possibly by altering intracellular vimentin localization, which is important for HBV cell entry. These results suggest that hzVSF has therapeutic potential for HBV infection with a novel mode of action. |
format | Online Article Text |
id | pubmed-8319007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83190072021-08-02 Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry Miyakawa, Yu Otsuka, Motoyuki Sekiba, Kazuma Funato, Kazuyoshi Koike, Kazuhiko Heliyon Research Article Although nucleos(t)ide analogs and interferons suppress hepatitis B virus (HBV) replication, they must be taken continuously and have a low response rate. Therefore, therapeutics for HBV with novel modes of action are needed. Humanized virus-suppressing factor (hzVSF) is a monoclonal antibody against vimentin that exhibits broad-spectrum antiviral activity. Here, hzVSF significantly inhibited HBV infection. Although hzVSF inhibited HBV RNA production, it did not affect viral transcription from minicircle DNA mimicking covalently closed circular DNA. Additionally, hzVSF did not inhibit viral protein or DNA release from infected cells. Rather, hzVSF inhibited the cell entry of viral preS1 peptides, possibly by altering intracellular vimentin localization, which is important for HBV cell entry. These results suggest that hzVSF has therapeutic potential for HBV infection with a novel mode of action. Elsevier 2021-07-14 /pmc/articles/PMC8319007/ /pubmed/34345745 http://dx.doi.org/10.1016/j.heliyon.2021.e07586 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Miyakawa, Yu Otsuka, Motoyuki Sekiba, Kazuma Funato, Kazuyoshi Koike, Kazuhiko Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title | Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title_full | Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title_fullStr | Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title_full_unstemmed | Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title_short | Humanized virus-suppressing factor inhibits hepatitis B virus infection by targeting viral cell entry |
title_sort | humanized virus-suppressing factor inhibits hepatitis b virus infection by targeting viral cell entry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319007/ https://www.ncbi.nlm.nih.gov/pubmed/34345745 http://dx.doi.org/10.1016/j.heliyon.2021.e07586 |
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