A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds

We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers t...

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Detalles Bibliográficos
Autores principales: Strobl, Frederic, Stelzer, Ernst H. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319312/
https://www.ncbi.nlm.nih.gov/pubmed/34321513
http://dx.doi.org/10.1038/s41598-021-94288-0
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author Strobl, Frederic
Stelzer, Ernst H. K.
author_facet Strobl, Frederic
Stelzer, Ernst H. K.
author_sort Strobl, Frederic
collection PubMed
description We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
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spelling pubmed-83193122021-07-29 A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds Strobl, Frederic Stelzer, Ernst H. K. Sci Rep Article We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings. Nature Publishing Group UK 2021-07-28 /pmc/articles/PMC8319312/ /pubmed/34321513 http://dx.doi.org/10.1038/s41598-021-94288-0 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Strobl, Frederic
Stelzer, Ernst H. K.
A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_full A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_fullStr A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_full_unstemmed A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_short A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
title_sort deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319312/
https://www.ncbi.nlm.nih.gov/pubmed/34321513
http://dx.doi.org/10.1038/s41598-021-94288-0
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