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Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever

Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restrict...

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Autores principales: Napoleão-Pêgo, Paloma, Carneiro, Flávia R. G., Durans, Andressa M., Gomes, Larissa R., Morel, Carlos M., Provance, David W., De-Simone, Salvatore G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319364/
https://www.ncbi.nlm.nih.gov/pubmed/34321560
http://dx.doi.org/10.1038/s41598-021-94817-x
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author Napoleão-Pêgo, Paloma
Carneiro, Flávia R. G.
Durans, Andressa M.
Gomes, Larissa R.
Morel, Carlos M.
Provance, David W.
De-Simone, Salvatore G.
author_facet Napoleão-Pêgo, Paloma
Carneiro, Flávia R. G.
Durans, Andressa M.
Gomes, Larissa R.
Morel, Carlos M.
Provance, David W.
De-Simone, Salvatore G.
author_sort Napoleão-Pêgo, Paloma
collection PubMed
description Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections.
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spelling pubmed-83193642021-07-29 Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever Napoleão-Pêgo, Paloma Carneiro, Flávia R. G. Durans, Andressa M. Gomes, Larissa R. Morel, Carlos M. Provance, David W. De-Simone, Salvatore G. Sci Rep Article Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections. Nature Publishing Group UK 2021-07-28 /pmc/articles/PMC8319364/ /pubmed/34321560 http://dx.doi.org/10.1038/s41598-021-94817-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Napoleão-Pêgo, Paloma
Carneiro, Flávia R. G.
Durans, Andressa M.
Gomes, Larissa R.
Morel, Carlos M.
Provance, David W.
De-Simone, Salvatore G.
Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_full Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_fullStr Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_full_unstemmed Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_short Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever
title_sort performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute mayaro fever
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319364/
https://www.ncbi.nlm.nih.gov/pubmed/34321560
http://dx.doi.org/10.1038/s41598-021-94817-x
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