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A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus
A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca(2+) signaling at single cell resolution in the present study. This microfluidic array consists of multiple radially aligned flow channels with equ...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319492/ https://www.ncbi.nlm.nih.gov/pubmed/34269374 http://dx.doi.org/10.1042/BSR20210719 |
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author | Wei, Wenbo Zhang, Miao Xu, Zhongyuan Li, Weifeng Cheng, Lixin Cao, Hongbao Ma, Min Chen, Zongzheng |
author_facet | Wei, Wenbo Zhang, Miao Xu, Zhongyuan Li, Weifeng Cheng, Lixin Cao, Hongbao Ma, Min Chen, Zongzheng |
author_sort | Wei, Wenbo |
collection | PubMed |
description | A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca(2+) signaling at single cell resolution in the present study. This microfluidic array consists of multiple radially aligned flow channels with equal intersection angles, which was designed by a combination of stagnation point flow and physical barrier. Numerical simulation results and trajectory analysis have shown the effectiveness of this single cell trapping device. Fluorescent experiment results demonstrated the effects of flow rate and frequency of dynamic stimulus on the profiles of biochemical concentration which exposed on captured cells. In this microarray, the captured single cells in each trapping channels were able to receive identical extracellular dynamic biochemical stimuli which being transmitted from the entrance in the middle of the microfluidic array. Besides, after loading dynamic Adenosine Triphosphate (ATP) stimulation on captured cells by this device, consistent average intracellular Ca(2+) dynamics phase and cellular heterogeneity were observed in captured single K562 cells. Furthermore, this device is able to be used for investigating cellular respond on single cell resolution to temporally varying environments by modulating the stimulation signal in terms of concentration, pattern, and duration of exposure. |
format | Online Article Text |
id | pubmed-8319492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83194922021-08-10 A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus Wei, Wenbo Zhang, Miao Xu, Zhongyuan Li, Weifeng Cheng, Lixin Cao, Hongbao Ma, Min Chen, Zongzheng Biosci Rep Bioenergetics A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca(2+) signaling at single cell resolution in the present study. This microfluidic array consists of multiple radially aligned flow channels with equal intersection angles, which was designed by a combination of stagnation point flow and physical barrier. Numerical simulation results and trajectory analysis have shown the effectiveness of this single cell trapping device. Fluorescent experiment results demonstrated the effects of flow rate and frequency of dynamic stimulus on the profiles of biochemical concentration which exposed on captured cells. In this microarray, the captured single cells in each trapping channels were able to receive identical extracellular dynamic biochemical stimuli which being transmitted from the entrance in the middle of the microfluidic array. Besides, after loading dynamic Adenosine Triphosphate (ATP) stimulation on captured cells by this device, consistent average intracellular Ca(2+) dynamics phase and cellular heterogeneity were observed in captured single K562 cells. Furthermore, this device is able to be used for investigating cellular respond on single cell resolution to temporally varying environments by modulating the stimulation signal in terms of concentration, pattern, and duration of exposure. Portland Press Ltd. 2021-07-28 /pmc/articles/PMC8319492/ /pubmed/34269374 http://dx.doi.org/10.1042/BSR20210719 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Bioenergetics Wei, Wenbo Zhang, Miao Xu, Zhongyuan Li, Weifeng Cheng, Lixin Cao, Hongbao Ma, Min Chen, Zongzheng A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title | A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title_full | A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title_fullStr | A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title_full_unstemmed | A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title_short | A microfluidic array device for single cell capture and intracellular Ca(2+) response analysis induced by dynamic biochemical stimulus |
title_sort | microfluidic array device for single cell capture and intracellular ca(2+) response analysis induced by dynamic biochemical stimulus |
topic | Bioenergetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319492/ https://www.ncbi.nlm.nih.gov/pubmed/34269374 http://dx.doi.org/10.1042/BSR20210719 |
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