Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell de...

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Detalles Bibliográficos
Autores principales: Agrawal, Ishan, Sharma, Nidhi, Saxena, Shivanjali, Arvind, S., Chakraborty, Debayani, Chakraborty, Debarati Bhunia, Jha, Deepak, Ghatak, Surajit, Epari, Sridhar, Gupta, Tejpal, Jha, Sushmita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319797/
https://www.ncbi.nlm.nih.gov/pubmed/34355202
http://dx.doi.org/10.1016/j.xpro.2021.100678
Descripción
Sumario:Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).

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