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Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell de...

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Autores principales: Agrawal, Ishan, Sharma, Nidhi, Saxena, Shivanjali, Arvind, S., Chakraborty, Debayani, Chakraborty, Debarati Bhunia, Jha, Deepak, Ghatak, Surajit, Epari, Sridhar, Gupta, Tejpal, Jha, Sushmita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319797/
https://www.ncbi.nlm.nih.gov/pubmed/34355202
http://dx.doi.org/10.1016/j.xpro.2021.100678
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author Agrawal, Ishan
Sharma, Nidhi
Saxena, Shivanjali
Arvind, S.
Chakraborty, Debayani
Chakraborty, Debarati Bhunia
Jha, Deepak
Ghatak, Surajit
Epari, Sridhar
Gupta, Tejpal
Jha, Sushmita
author_facet Agrawal, Ishan
Sharma, Nidhi
Saxena, Shivanjali
Arvind, S.
Chakraborty, Debayani
Chakraborty, Debarati Bhunia
Jha, Deepak
Ghatak, Surajit
Epari, Sridhar
Gupta, Tejpal
Jha, Sushmita
author_sort Agrawal, Ishan
collection PubMed
description Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).
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spelling pubmed-83197972021-08-04 Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells Agrawal, Ishan Sharma, Nidhi Saxena, Shivanjali Arvind, S. Chakraborty, Debayani Chakraborty, Debarati Bhunia Jha, Deepak Ghatak, Surajit Epari, Sridhar Gupta, Tejpal Jha, Sushmita STAR Protoc Protocol Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021). Elsevier 2021-07-20 /pmc/articles/PMC8319797/ /pubmed/34355202 http://dx.doi.org/10.1016/j.xpro.2021.100678 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Agrawal, Ishan
Sharma, Nidhi
Saxena, Shivanjali
Arvind, S.
Chakraborty, Debayani
Chakraborty, Debarati Bhunia
Jha, Deepak
Ghatak, Surajit
Epari, Sridhar
Gupta, Tejpal
Jha, Sushmita
Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title_full Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title_fullStr Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title_full_unstemmed Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title_short Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
title_sort protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319797/
https://www.ncbi.nlm.nih.gov/pubmed/34355202
http://dx.doi.org/10.1016/j.xpro.2021.100678
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