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Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell de...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319797/ https://www.ncbi.nlm.nih.gov/pubmed/34355202 http://dx.doi.org/10.1016/j.xpro.2021.100678 |
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author | Agrawal, Ishan Sharma, Nidhi Saxena, Shivanjali Arvind, S. Chakraborty, Debayani Chakraborty, Debarati Bhunia Jha, Deepak Ghatak, Surajit Epari, Sridhar Gupta, Tejpal Jha, Sushmita |
author_facet | Agrawal, Ishan Sharma, Nidhi Saxena, Shivanjali Arvind, S. Chakraborty, Debayani Chakraborty, Debarati Bhunia Jha, Deepak Ghatak, Surajit Epari, Sridhar Gupta, Tejpal Jha, Sushmita |
author_sort | Agrawal, Ishan |
collection | PubMed |
description | Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021). |
format | Online Article Text |
id | pubmed-8319797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83197972021-08-04 Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells Agrawal, Ishan Sharma, Nidhi Saxena, Shivanjali Arvind, S. Chakraborty, Debayani Chakraborty, Debarati Bhunia Jha, Deepak Ghatak, Surajit Epari, Sridhar Gupta, Tejpal Jha, Sushmita STAR Protoc Protocol Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021). Elsevier 2021-07-20 /pmc/articles/PMC8319797/ /pubmed/34355202 http://dx.doi.org/10.1016/j.xpro.2021.100678 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Agrawal, Ishan Sharma, Nidhi Saxena, Shivanjali Arvind, S. Chakraborty, Debayani Chakraborty, Debarati Bhunia Jha, Deepak Ghatak, Surajit Epari, Sridhar Gupta, Tejpal Jha, Sushmita Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title_full | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title_fullStr | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title_full_unstemmed | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title_short | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
title_sort | protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319797/ https://www.ncbi.nlm.nih.gov/pubmed/34355202 http://dx.doi.org/10.1016/j.xpro.2021.100678 |
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