Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single imm...

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Detalles Bibliográficos
Autores principales: Hagen, Sven Hendrik, Hennesen, Jana, Altfeld, Marcus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319808/
https://www.ncbi.nlm.nih.gov/pubmed/34355200
http://dx.doi.org/10.1016/j.xpro.2021.100641
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author Hagen, Sven Hendrik
Hennesen, Jana
Altfeld, Marcus
author_facet Hagen, Sven Hendrik
Hennesen, Jana
Altfeld, Marcus
author_sort Hagen, Sven Hendrik
collection PubMed
description X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single immune cell level using a single-nucleotide polymorphism approach. The protocol furthermore allows the analysis of allele-specific expression of X-chromosomal genes. For complete details on the use and execution of this protocol, please refer to Hagen et al. (2020).
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spelling pubmed-83198082021-08-04 Assessment of escape from X chromosome inactivation and gene expression in single human immune cells Hagen, Sven Hendrik Hennesen, Jana Altfeld, Marcus STAR Protoc Protocol X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single immune cell level using a single-nucleotide polymorphism approach. The protocol furthermore allows the analysis of allele-specific expression of X-chromosomal genes. For complete details on the use and execution of this protocol, please refer to Hagen et al. (2020). Elsevier 2021-07-20 /pmc/articles/PMC8319808/ /pubmed/34355200 http://dx.doi.org/10.1016/j.xpro.2021.100641 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Hagen, Sven Hendrik
Hennesen, Jana
Altfeld, Marcus
Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title_full Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title_fullStr Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title_full_unstemmed Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title_short Assessment of escape from X chromosome inactivation and gene expression in single human immune cells
title_sort assessment of escape from x chromosome inactivation and gene expression in single human immune cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319808/
https://www.ncbi.nlm.nih.gov/pubmed/34355200
http://dx.doi.org/10.1016/j.xpro.2021.100641
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