How to Minimize Light–Organic Matter Interactions for All-Optical Sub-Cutaneous Temperature Sensing

[Image: see text] Penetration and emanation of light into tissue are limited by the strong interaction of light with the tissue components, especially oxygenated hemoglobin and white adipose tissue. This limits the possibilities for all-optical minimal invasive sensing. In order to minimize the optical losses of light in and out of the tissue, only a narrow optical window between 630 and 900 nm is available. In this work, we realized for the first time all-optical temperature sensing within the narrow optical window for tissue by using the process of triplet–triplet annihilation photon energy upconversion (TTA-UC) as a sensing tool. For this, we apply the asymmetrical benzo-fused BODIPY dye as an optimal emitter and mixed palladium benzo-naphtho-porphyrins as an optimal sensitizer. The TTA-UC sensing system is excited with λ = 658 nm with an extremely low intensity of 1 mW × cm(–2) and is factual-protected for a time period longer than 100 s against oxygen-stimulated damage, allowing a stable demonstration of this T-sensing system also in an oxygen-rich environment without losing sensitivity. The sensing dyes we embed in the natural wax/natural matrix, which is intrinsically biocompatible, are approved by the FDA as food additives. The demonstrated temperature sensitivity is higher than ΔT = 200 mK placed around the physiologically relevant temperature of T = 36 °C.