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Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase
Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activiti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320891/ https://www.ncbi.nlm.nih.gov/pubmed/34336800 http://dx.doi.org/10.3389/fbioe.2021.681253 |
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author | Choi, Mi Na Shin, Kyung-Chul Kim, Dae Wook Kim, Baek-Joong Park, Chang-Su Yeom, Soo-Jin Kim, Yeong-Su |
author_facet | Choi, Mi Na Shin, Kyung-Chul Kim, Dae Wook Kim, Baek-Joong Park, Chang-Su Yeom, Soo-Jin Kim, Yeong-Su |
author_sort | Choi, Mi Na |
collection | PubMed |
description | Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose. |
format | Online Article Text |
id | pubmed-8320891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83208912021-07-30 Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase Choi, Mi Na Shin, Kyung-Chul Kim, Dae Wook Kim, Baek-Joong Park, Chang-Su Yeom, Soo-Jin Kim, Yeong-Su Front Bioeng Biotechnol Bioengineering and Biotechnology Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose. Frontiers Media S.A. 2021-07-15 /pmc/articles/PMC8320891/ /pubmed/34336800 http://dx.doi.org/10.3389/fbioe.2021.681253 Text en Copyright © 2021 Choi, Shin, Kim, Kim, Park, Yeom and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Choi, Mi Na Shin, Kyung-Chul Kim, Dae Wook Kim, Baek-Joong Park, Chang-Su Yeom, Soo-Jin Kim, Yeong-Su Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title | Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title_full | Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title_fullStr | Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title_full_unstemmed | Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title_short | Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase |
title_sort | production of d-allose from d-allulose using commercial immobilized glucose isomerase |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320891/ https://www.ncbi.nlm.nih.gov/pubmed/34336800 http://dx.doi.org/10.3389/fbioe.2021.681253 |
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