Cargando…

Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus

Invasive species present a worldwide concern as competition and pathogen reservoirs for native species. Specifically, the invasive social wasp, Vespula pensylvanica, is native to western North America and has become naturalized in Hawaii, where it exerts pressures on native arthropod communities as...

Descripción completa

Detalles Bibliográficos
Autores principales: Rothman, Jason A., Loope, Kevin J., McFrederick, Quinn S., Wilson Rankin, Erin E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321129/
https://www.ncbi.nlm.nih.gov/pubmed/34324610
http://dx.doi.org/10.1371/journal.pone.0255463
_version_ 1783730778304151552
author Rothman, Jason A.
Loope, Kevin J.
McFrederick, Quinn S.
Wilson Rankin, Erin E.
author_facet Rothman, Jason A.
Loope, Kevin J.
McFrederick, Quinn S.
Wilson Rankin, Erin E.
author_sort Rothman, Jason A.
collection PubMed
description Invasive species present a worldwide concern as competition and pathogen reservoirs for native species. Specifically, the invasive social wasp, Vespula pensylvanica, is native to western North America and has become naturalized in Hawaii, where it exerts pressures on native arthropod communities as a competitor and predator. As invasive species may alter the microbial and disease ecology of their introduced ranges, there is a need to understand the microbiomes and virology of social wasps. We used 16S rRNA gene sequencing to characterize the microbiome of V. pensylvanica samples pooled by colony across two geographically distinct ranges and found that wasps generally associate with taxa within the bacterial genera Fructobacillus, Fructilactobacillus, Lactococcus, Leuconostoc, and Zymobacter, and likely associate with environmentally-acquired bacteria. Furthermore, V. pensylvanica harbors—and in some cases were dominated by—many endosymbionts including Wolbachia, Sodalis, Arsenophonus, and Rickettsia, and were found to contain bee-associated taxa, likely due to scavenging on or predation upon honey bees. Next, we used reverse-transcriptase quantitative PCR to assay colony-level infection intensity for Moku virus (family: Iflaviridae), a recently-described disease that is known to infect multiple Hymenopteran species. While Moku virus was prevalent and in high titer, it did not associate with microbial diversity, indicating that the microbiome may not directly interact with Moku virus in V. pensylvanica in meaningful ways. Collectively, our results suggest that the invasive social wasp V. pensylvanica associates with a simple microbiome, may be infected with putative endosymbionts, likely acquires bacterial taxa from the environment and diet, and is often infected with Moku virus. Our results suggest that V. pensylvanica, like other invasive social insects, has the potential to act as a reservoir for bacteria pathogenic to other pollinators, though this requires experimental demonstration.
format Online
Article
Text
id pubmed-8321129
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-83211292021-07-31 Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus Rothman, Jason A. Loope, Kevin J. McFrederick, Quinn S. Wilson Rankin, Erin E. PLoS One Research Article Invasive species present a worldwide concern as competition and pathogen reservoirs for native species. Specifically, the invasive social wasp, Vespula pensylvanica, is native to western North America and has become naturalized in Hawaii, where it exerts pressures on native arthropod communities as a competitor and predator. As invasive species may alter the microbial and disease ecology of their introduced ranges, there is a need to understand the microbiomes and virology of social wasps. We used 16S rRNA gene sequencing to characterize the microbiome of V. pensylvanica samples pooled by colony across two geographically distinct ranges and found that wasps generally associate with taxa within the bacterial genera Fructobacillus, Fructilactobacillus, Lactococcus, Leuconostoc, and Zymobacter, and likely associate with environmentally-acquired bacteria. Furthermore, V. pensylvanica harbors—and in some cases were dominated by—many endosymbionts including Wolbachia, Sodalis, Arsenophonus, and Rickettsia, and were found to contain bee-associated taxa, likely due to scavenging on or predation upon honey bees. Next, we used reverse-transcriptase quantitative PCR to assay colony-level infection intensity for Moku virus (family: Iflaviridae), a recently-described disease that is known to infect multiple Hymenopteran species. While Moku virus was prevalent and in high titer, it did not associate with microbial diversity, indicating that the microbiome may not directly interact with Moku virus in V. pensylvanica in meaningful ways. Collectively, our results suggest that the invasive social wasp V. pensylvanica associates with a simple microbiome, may be infected with putative endosymbionts, likely acquires bacterial taxa from the environment and diet, and is often infected with Moku virus. Our results suggest that V. pensylvanica, like other invasive social insects, has the potential to act as a reservoir for bacteria pathogenic to other pollinators, though this requires experimental demonstration. Public Library of Science 2021-07-29 /pmc/articles/PMC8321129/ /pubmed/34324610 http://dx.doi.org/10.1371/journal.pone.0255463 Text en © 2021 Rothman et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rothman, Jason A.
Loope, Kevin J.
McFrederick, Quinn S.
Wilson Rankin, Erin E.
Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title_full Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title_fullStr Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title_full_unstemmed Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title_short Microbiome of the wasp Vespula pensylvanica in native and invasive populations, and associations with Moku virus
title_sort microbiome of the wasp vespula pensylvanica in native and invasive populations, and associations with moku virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321129/
https://www.ncbi.nlm.nih.gov/pubmed/34324610
http://dx.doi.org/10.1371/journal.pone.0255463
work_keys_str_mv AT rothmanjasona microbiomeofthewaspvespulapensylvanicainnativeandinvasivepopulationsandassociationswithmokuvirus
AT loopekevinj microbiomeofthewaspvespulapensylvanicainnativeandinvasivepopulationsandassociationswithmokuvirus
AT mcfrederickquinns microbiomeofthewaspvespulapensylvanicainnativeandinvasivepopulationsandassociationswithmokuvirus
AT wilsonrankinerine microbiomeofthewaspvespulapensylvanicainnativeandinvasivepopulationsandassociationswithmokuvirus