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Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study

Naso-pharyngeal RT-PCR is the gold standard for the diagnosis of COVID-19, but there is a need for rapid and reliable tests. Some validation studies have used frozen aliquots mainly from adults. The aim of this real-life study was to test the performance of a SARS-CoV-2 rapid antigen test (SC2-RAT)...

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Autores principales: Jung, Camille, Levy, Corinne, Varon, Emmanuelle, Biscardi, Sandra, Batard, Christophe, Wollner, Alain, Deberdt, Patrice, Sellam, Aurélie, Hau, Isabelle, Cohen, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321236/
https://www.ncbi.nlm.nih.gov/pubmed/34336732
http://dx.doi.org/10.3389/fped.2021.647274
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author Jung, Camille
Levy, Corinne
Varon, Emmanuelle
Biscardi, Sandra
Batard, Christophe
Wollner, Alain
Deberdt, Patrice
Sellam, Aurélie
Hau, Isabelle
Cohen, Robert
author_facet Jung, Camille
Levy, Corinne
Varon, Emmanuelle
Biscardi, Sandra
Batard, Christophe
Wollner, Alain
Deberdt, Patrice
Sellam, Aurélie
Hau, Isabelle
Cohen, Robert
author_sort Jung, Camille
collection PubMed
description Naso-pharyngeal RT-PCR is the gold standard for the diagnosis of COVID-19, but there is a need for rapid and reliable tests. Some validation studies have used frozen aliquots mainly from adults. The aim of this real-life study was to test the performance of a SARS-CoV-2 rapid antigen test (SC2-RAT) in children. Symptomatic patients aged 0 to 17 years were recruited in the emergency department of the University Hospital of Creteil and in primary care pediatric practices from October 10, 2020 for 7 weeks. Each enrolled child had a SARS-CoV-2 RT-PCR test and a SC2-RAT from two distinct nasopharyngeal swabs. Among the 308 patients (mean [SD] age 4.9 [5.3] years), fever was the main symptom (73.4%), with no difference between COVID-19–negative and –positive groups. The prevalence of COVID-19 was 10.7% (95% CI 7.5–14.7). On the whole cohort, the sensitivity and specificity of the SC2-RAT compared to RT-PCR was 87.9% (95% CI 71.8–96.6) and 98.5% (95% CI 96.3–99.6). Considering samples with cycle threshold >25, the sensibility was lower: 63.6% (95% CI 30.8–89.1) and the specificity 99.6% (95% CI 98.0–100.0). The mean delay to obtain an SC2-RAT result was <15 min but was 3.2 h (SD 5.5) for an RT-PCR result. Contact with a COVID-19–positive person was more frequent for COVID-19–positive than –negative patients (n = 21, 61.6%, vs. n = 64, 24.6%; p < 0.01). In real life, SC2-RAT seems reliable for symptomatic children, allowing to detect contagious children.
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spelling pubmed-83212362021-07-30 Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study Jung, Camille Levy, Corinne Varon, Emmanuelle Biscardi, Sandra Batard, Christophe Wollner, Alain Deberdt, Patrice Sellam, Aurélie Hau, Isabelle Cohen, Robert Front Pediatr Pediatrics Naso-pharyngeal RT-PCR is the gold standard for the diagnosis of COVID-19, but there is a need for rapid and reliable tests. Some validation studies have used frozen aliquots mainly from adults. The aim of this real-life study was to test the performance of a SARS-CoV-2 rapid antigen test (SC2-RAT) in children. Symptomatic patients aged 0 to 17 years were recruited in the emergency department of the University Hospital of Creteil and in primary care pediatric practices from October 10, 2020 for 7 weeks. Each enrolled child had a SARS-CoV-2 RT-PCR test and a SC2-RAT from two distinct nasopharyngeal swabs. Among the 308 patients (mean [SD] age 4.9 [5.3] years), fever was the main symptom (73.4%), with no difference between COVID-19–negative and –positive groups. The prevalence of COVID-19 was 10.7% (95% CI 7.5–14.7). On the whole cohort, the sensitivity and specificity of the SC2-RAT compared to RT-PCR was 87.9% (95% CI 71.8–96.6) and 98.5% (95% CI 96.3–99.6). Considering samples with cycle threshold >25, the sensibility was lower: 63.6% (95% CI 30.8–89.1) and the specificity 99.6% (95% CI 98.0–100.0). The mean delay to obtain an SC2-RAT result was <15 min but was 3.2 h (SD 5.5) for an RT-PCR result. Contact with a COVID-19–positive person was more frequent for COVID-19–positive than –negative patients (n = 21, 61.6%, vs. n = 64, 24.6%; p < 0.01). In real life, SC2-RAT seems reliable for symptomatic children, allowing to detect contagious children. Frontiers Media S.A. 2021-07-15 /pmc/articles/PMC8321236/ /pubmed/34336732 http://dx.doi.org/10.3389/fped.2021.647274 Text en Copyright © 2021 Jung, Levy, Varon, Biscardi, Batard, Wollner, Deberdt, Sellam, Hau and Cohen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Jung, Camille
Levy, Corinne
Varon, Emmanuelle
Biscardi, Sandra
Batard, Christophe
Wollner, Alain
Deberdt, Patrice
Sellam, Aurélie
Hau, Isabelle
Cohen, Robert
Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title_full Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title_fullStr Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title_full_unstemmed Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title_short Diagnostic Accuracy of SARS-CoV-2 Antigen Detection Test in Children: A Real-Life Study
title_sort diagnostic accuracy of sars-cov-2 antigen detection test in children: a real-life study
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321236/
https://www.ncbi.nlm.nih.gov/pubmed/34336732
http://dx.doi.org/10.3389/fped.2021.647274
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