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RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Physiological Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321853/ https://www.ncbi.nlm.nih.gov/pubmed/34010080 http://dx.doi.org/10.1152/ajplung.00118.2021 |
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author | Linfield, Debra T. Gao, Nannan Raduka, Andjela Harford, Terri J. Piedimonte, Giovanni Rezaee, Fariba |
author_facet | Linfield, Debra T. Gao, Nannan Raduka, Andjela Harford, Terri J. Piedimonte, Giovanni Rezaee, Fariba |
author_sort | Linfield, Debra T. |
collection | PubMed |
description | The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine whether RSV delays the airway epithelium wound repair process both in vitro and in vivo. We found that RSV infection attenuated epithelial cell migration, a step in wound repair, promoted stress fiber formation, and mediated assembly of large focal adhesions. Inhibition of Rho-associated kinase, a master regulator of actin function, reversed these effects. There was increased RhoA and phospho-myosin light chain 2 following RSV infection. In vivo, mice were intraperitoneally inoculated with naphthalene to induce lung injury, followed by RSV infection. RSV infection delayed reepithelialization. There were increased concentrations of phospho-myosin light chain 2 in day 7 naphthalene + RSV animals, which normalized by day 14. This study suggests a key mechanism by which RSV infection delays wound healing. |
format | Online Article Text |
id | pubmed-8321853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Physiological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-83218532022-07-01 RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration Linfield, Debra T. Gao, Nannan Raduka, Andjela Harford, Terri J. Piedimonte, Giovanni Rezaee, Fariba Am J Physiol Lung Cell Mol Physiol Research Article The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine whether RSV delays the airway epithelium wound repair process both in vitro and in vivo. We found that RSV infection attenuated epithelial cell migration, a step in wound repair, promoted stress fiber formation, and mediated assembly of large focal adhesions. Inhibition of Rho-associated kinase, a master regulator of actin function, reversed these effects. There was increased RhoA and phospho-myosin light chain 2 following RSV infection. In vivo, mice were intraperitoneally inoculated with naphthalene to induce lung injury, followed by RSV infection. RSV infection delayed reepithelialization. There were increased concentrations of phospho-myosin light chain 2 in day 7 naphthalene + RSV animals, which normalized by day 14. This study suggests a key mechanism by which RSV infection delays wound healing. American Physiological Society 2021-07-01 2021-05-19 /pmc/articles/PMC8321853/ /pubmed/34010080 http://dx.doi.org/10.1152/ajplung.00118.2021 Text en Copyright © 2021 The Authors https://creativecommons.org/licenses/by/4.0/Licensed under Creative Commons Attribution CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/) . Published by the American Physiological Society. |
spellingShingle | Research Article Linfield, Debra T. Gao, Nannan Raduka, Andjela Harford, Terri J. Piedimonte, Giovanni Rezaee, Fariba RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title | RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title_full | RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title_fullStr | RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title_full_unstemmed | RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title_short | RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
title_sort | rsv attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321853/ https://www.ncbi.nlm.nih.gov/pubmed/34010080 http://dx.doi.org/10.1152/ajplung.00118.2021 |
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