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RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration

The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine...

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Autores principales: Linfield, Debra T., Gao, Nannan, Raduka, Andjela, Harford, Terri J., Piedimonte, Giovanni, Rezaee, Fariba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321853/
https://www.ncbi.nlm.nih.gov/pubmed/34010080
http://dx.doi.org/10.1152/ajplung.00118.2021
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author Linfield, Debra T.
Gao, Nannan
Raduka, Andjela
Harford, Terri J.
Piedimonte, Giovanni
Rezaee, Fariba
author_facet Linfield, Debra T.
Gao, Nannan
Raduka, Andjela
Harford, Terri J.
Piedimonte, Giovanni
Rezaee, Fariba
author_sort Linfield, Debra T.
collection PubMed
description The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine whether RSV delays the airway epithelium wound repair process both in vitro and in vivo. We found that RSV infection attenuated epithelial cell migration, a step in wound repair, promoted stress fiber formation, and mediated assembly of large focal adhesions. Inhibition of Rho-associated kinase, a master regulator of actin function, reversed these effects. There was increased RhoA and phospho-myosin light chain 2 following RSV infection. In vivo, mice were intraperitoneally inoculated with naphthalene to induce lung injury, followed by RSV infection. RSV infection delayed reepithelialization. There were increased concentrations of phospho-myosin light chain 2 in day 7 naphthalene + RSV animals, which normalized by day 14. This study suggests a key mechanism by which RSV infection delays wound healing.
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spelling pubmed-83218532022-07-01 RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration Linfield, Debra T. Gao, Nannan Raduka, Andjela Harford, Terri J. Piedimonte, Giovanni Rezaee, Fariba Am J Physiol Lung Cell Mol Physiol Research Article The airway epithelium’s ability to repair itself after injury, known as epithelial restitution, is an essential mechanism enabling the respiratory tract’s normal functions. Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections worldwide. We sought to determine whether RSV delays the airway epithelium wound repair process both in vitro and in vivo. We found that RSV infection attenuated epithelial cell migration, a step in wound repair, promoted stress fiber formation, and mediated assembly of large focal adhesions. Inhibition of Rho-associated kinase, a master regulator of actin function, reversed these effects. There was increased RhoA and phospho-myosin light chain 2 following RSV infection. In vivo, mice were intraperitoneally inoculated with naphthalene to induce lung injury, followed by RSV infection. RSV infection delayed reepithelialization. There were increased concentrations of phospho-myosin light chain 2 in day 7 naphthalene + RSV animals, which normalized by day 14. This study suggests a key mechanism by which RSV infection delays wound healing. American Physiological Society 2021-07-01 2021-05-19 /pmc/articles/PMC8321853/ /pubmed/34010080 http://dx.doi.org/10.1152/ajplung.00118.2021 Text en Copyright © 2021 The Authors https://creativecommons.org/licenses/by/4.0/Licensed under Creative Commons Attribution CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/) . Published by the American Physiological Society.
spellingShingle Research Article
Linfield, Debra T.
Gao, Nannan
Raduka, Andjela
Harford, Terri J.
Piedimonte, Giovanni
Rezaee, Fariba
RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title_full RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title_fullStr RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title_full_unstemmed RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title_short RSV attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
title_sort rsv attenuates epithelial cell restitution by inhibiting actin cytoskeleton-dependent cell migration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321853/
https://www.ncbi.nlm.nih.gov/pubmed/34010080
http://dx.doi.org/10.1152/ajplung.00118.2021
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