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Larval cryopreservation as new management tool for threatened clam fisheries
Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively affected...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322048/ https://www.ncbi.nlm.nih.gov/pubmed/34326357 http://dx.doi.org/10.1038/s41598-021-94197-2 |
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author | Heres, P. Troncoso, J. Paredes, E. |
author_facet | Heres, P. Troncoso, J. Paredes, E. |
author_sort | Heres, P. |
collection | PubMed |
description | Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively affected the populations of high economical value shellfish in Spain and, as a result, many are declining or threatened nowadays. This study was focused on early-life stages of Venerupis corrugata, Ruditapes decussatus and Ruditapes philippinarum to develop successful protocols to enhance the conservation effort and sustainable shellfishery resources. Firstly, common cryoprotecting agents (CPAs) were tested to select the suitable permeable CPA attending to toxicity. Cryopreservation success using different combinations of CPA solutions, increasing equilibrium times and larval stages was evaluated attending to survival and shell growth at 2 days post-thawing. Older clam development stages were more tolerant to CPA toxicity, being ethylene-glycol (EG) and Propylene-glycol (PG) the least toxic CPAs. CPA solution containing EG yielded the highest post-thawing survival rate and the increase of equilibration time was not beneficial for clam larvae. Cryopreservation of trochophores yielded around 50% survivorship, whereas over 80% of cryopreserved D-larvae were able to recover after thawing. |
format | Online Article Text |
id | pubmed-8322048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83220482021-07-30 Larval cryopreservation as new management tool for threatened clam fisheries Heres, P. Troncoso, J. Paredes, E. Sci Rep Article Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively affected the populations of high economical value shellfish in Spain and, as a result, many are declining or threatened nowadays. This study was focused on early-life stages of Venerupis corrugata, Ruditapes decussatus and Ruditapes philippinarum to develop successful protocols to enhance the conservation effort and sustainable shellfishery resources. Firstly, common cryoprotecting agents (CPAs) were tested to select the suitable permeable CPA attending to toxicity. Cryopreservation success using different combinations of CPA solutions, increasing equilibrium times and larval stages was evaluated attending to survival and shell growth at 2 days post-thawing. Older clam development stages were more tolerant to CPA toxicity, being ethylene-glycol (EG) and Propylene-glycol (PG) the least toxic CPAs. CPA solution containing EG yielded the highest post-thawing survival rate and the increase of equilibration time was not beneficial for clam larvae. Cryopreservation of trochophores yielded around 50% survivorship, whereas over 80% of cryopreserved D-larvae were able to recover after thawing. Nature Publishing Group UK 2021-07-29 /pmc/articles/PMC8322048/ /pubmed/34326357 http://dx.doi.org/10.1038/s41598-021-94197-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Heres, P. Troncoso, J. Paredes, E. Larval cryopreservation as new management tool for threatened clam fisheries |
title | Larval cryopreservation as new management tool for threatened clam fisheries |
title_full | Larval cryopreservation as new management tool for threatened clam fisheries |
title_fullStr | Larval cryopreservation as new management tool for threatened clam fisheries |
title_full_unstemmed | Larval cryopreservation as new management tool for threatened clam fisheries |
title_short | Larval cryopreservation as new management tool for threatened clam fisheries |
title_sort | larval cryopreservation as new management tool for threatened clam fisheries |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322048/ https://www.ncbi.nlm.nih.gov/pubmed/34326357 http://dx.doi.org/10.1038/s41598-021-94197-2 |
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