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Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)

In China, the low egg production rate is a major challenge to Muscovy duck farmers. Hypothalamus and ovary play essential role in egg production of birds. However, there are little or no reports from these tissues to identify potential candidate genes responsible for egg production in White Muscovy...

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Autores principales: Bello, Semiu Folaniyi, Xu, Haiping, Guo, Lijin, Li, Kan, Zheng, Ming, Xu, Yibin, Zhang, Siyu, Bekele, Endashaw Jebessa, Bahareldin, Ali Abdalla, Zhu, Weijian, Zhang, Dexiang, Zhang, Xiquan, Ji, Congliang, Nie, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322464/
https://www.ncbi.nlm.nih.gov/pubmed/34298381
http://dx.doi.org/10.1016/j.psj.2021.101310
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author Bello, Semiu Folaniyi
Xu, Haiping
Guo, Lijin
Li, Kan
Zheng, Ming
Xu, Yibin
Zhang, Siyu
Bekele, Endashaw Jebessa
Bahareldin, Ali Abdalla
Zhu, Weijian
Zhang, Dexiang
Zhang, Xiquan
Ji, Congliang
Nie, Qinghua
author_facet Bello, Semiu Folaniyi
Xu, Haiping
Guo, Lijin
Li, Kan
Zheng, Ming
Xu, Yibin
Zhang, Siyu
Bekele, Endashaw Jebessa
Bahareldin, Ali Abdalla
Zhu, Weijian
Zhang, Dexiang
Zhang, Xiquan
Ji, Congliang
Nie, Qinghua
author_sort Bello, Semiu Folaniyi
collection PubMed
description In China, the low egg production rate is a major challenge to Muscovy duck farmers. Hypothalamus and ovary play essential role in egg production of birds. However, there are little or no reports from these tissues to identify potential candidate genes responsible for egg production in White Muscovy ducks. A total of 1,537 laying ducks were raised; the egg production traits which include age at first egg (days), number of eggs at 300 d, and number of eggs at 59 wk were recorded. Moreover, 4 lowest (LP) and 4 highest producing (HP) were selected at 59 wk of age, respectively. To understand the mechanism of egg laying regulation, we sequenced the hypothalamus and ovary transcriptome profiles in LP and HP using RNA-Seq. The results showed that the number of eggs at 300 d and number of eggs at 59 wk in the HP were significantly more (P < 0.001) than the LP ducks. In total, 106.98G clean bases were generated from 16 libraries with an average of 6.68G clean bases for each library. Further analysis showed 569 and 2,259 differentially expressed genes (DEGs) were identified in the hypothalamus and ovary between LP and HP, respectively. The KEGG pathway enrichment analysis revealed 114 and 139 pathways in the hypothalamus and ovary, respectively which includes Calcium signaling pathway, ECM-receptor interaction, Focal adhesion, MAPK signaling pathway, Apoptosis and Apelin signaling pathways that are involved in egg production. Based on the GO terms and KEGG pathways results, 10 potential candidate genes (P2RX1, LPAR2, ADORA1, FN1, AKT3, ADCY5, ADCY8, MAP3K8, PXN, and PTTG1) were identified to be responsible for egg production. Further, protein-protein interaction was analyzed to show the relationship between these candidate genes. Therefore, this study provides useful information on transcriptome of hypothalamus and ovary of LP and HP Muscovy ducks.
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spelling pubmed-83224642021-08-04 Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata) Bello, Semiu Folaniyi Xu, Haiping Guo, Lijin Li, Kan Zheng, Ming Xu, Yibin Zhang, Siyu Bekele, Endashaw Jebessa Bahareldin, Ali Abdalla Zhu, Weijian Zhang, Dexiang Zhang, Xiquan Ji, Congliang Nie, Qinghua Poult Sci PHYSIOLOGY AND REPRODUCTION In China, the low egg production rate is a major challenge to Muscovy duck farmers. Hypothalamus and ovary play essential role in egg production of birds. However, there are little or no reports from these tissues to identify potential candidate genes responsible for egg production in White Muscovy ducks. A total of 1,537 laying ducks were raised; the egg production traits which include age at first egg (days), number of eggs at 300 d, and number of eggs at 59 wk were recorded. Moreover, 4 lowest (LP) and 4 highest producing (HP) were selected at 59 wk of age, respectively. To understand the mechanism of egg laying regulation, we sequenced the hypothalamus and ovary transcriptome profiles in LP and HP using RNA-Seq. The results showed that the number of eggs at 300 d and number of eggs at 59 wk in the HP were significantly more (P < 0.001) than the LP ducks. In total, 106.98G clean bases were generated from 16 libraries with an average of 6.68G clean bases for each library. Further analysis showed 569 and 2,259 differentially expressed genes (DEGs) were identified in the hypothalamus and ovary between LP and HP, respectively. The KEGG pathway enrichment analysis revealed 114 and 139 pathways in the hypothalamus and ovary, respectively which includes Calcium signaling pathway, ECM-receptor interaction, Focal adhesion, MAPK signaling pathway, Apoptosis and Apelin signaling pathways that are involved in egg production. Based on the GO terms and KEGG pathways results, 10 potential candidate genes (P2RX1, LPAR2, ADORA1, FN1, AKT3, ADCY5, ADCY8, MAP3K8, PXN, and PTTG1) were identified to be responsible for egg production. Further, protein-protein interaction was analyzed to show the relationship between these candidate genes. Therefore, this study provides useful information on transcriptome of hypothalamus and ovary of LP and HP Muscovy ducks. Elsevier 2021-06-06 /pmc/articles/PMC8322464/ /pubmed/34298381 http://dx.doi.org/10.1016/j.psj.2021.101310 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle PHYSIOLOGY AND REPRODUCTION
Bello, Semiu Folaniyi
Xu, Haiping
Guo, Lijin
Li, Kan
Zheng, Ming
Xu, Yibin
Zhang, Siyu
Bekele, Endashaw Jebessa
Bahareldin, Ali Abdalla
Zhu, Weijian
Zhang, Dexiang
Zhang, Xiquan
Ji, Congliang
Nie, Qinghua
Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title_full Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title_fullStr Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title_full_unstemmed Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title_short Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white Muscovy ducks (Cairina moschata)
title_sort hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of white muscovy ducks (cairina moschata)
topic PHYSIOLOGY AND REPRODUCTION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322464/
https://www.ncbi.nlm.nih.gov/pubmed/34298381
http://dx.doi.org/10.1016/j.psj.2021.101310
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