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Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus

Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the...

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Autores principales: Slobodkina, Galina, Allioux, Maxime, Merkel, Alexander, Cambon-Bonavita, Marie-Anne, Alain, Karine, Jebbar, Mohamed, Slobodkin, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322695/
https://www.ncbi.nlm.nih.gov/pubmed/34335500
http://dx.doi.org/10.3389/fmicb.2021.679245
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author Slobodkina, Galina
Allioux, Maxime
Merkel, Alexander
Cambon-Bonavita, Marie-Anne
Alain, Karine
Jebbar, Mohamed
Slobodkin, Alexander
author_facet Slobodkina, Galina
Allioux, Maxime
Merkel, Alexander
Cambon-Bonavita, Marie-Anne
Alain, Karine
Jebbar, Mohamed
Slobodkin, Alexander
author_sort Slobodkina, Galina
collection PubMed
description Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56(T) (=DSM 110954(T) = VKM B-3474(T)). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56(T) grows lithoautotrophically with H(2) as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO(2)/HCO(3)(−) as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO(2) fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification.
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spelling pubmed-83226952021-07-31 Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus Slobodkina, Galina Allioux, Maxime Merkel, Alexander Cambon-Bonavita, Marie-Anne Alain, Karine Jebbar, Mohamed Slobodkin, Alexander Front Microbiol Microbiology Hyperthermophilic archaea of the genus Archaeoglobus are the subject of many fundamental and biotechnological researches. Despite their significance, the class Archaeoglobi is currently represented by only eight species obtained as axenic cultures and taxonomically characterized. Here, we report the isolation and characterization of a new species of Archaeoglobus from a deep-sea hydrothermal vent (Mid-Atlantic Ridge, TAG) for which the name Archaeoglobus neptunius sp. nov. is proposed. The type strain is SE56(T) (=DSM 110954(T) = VKM B-3474(T)). The cells of the novel isolate are motile irregular cocci growing at 50–85°C, pH 5.5–7.5, and NaCl concentrations of 1.5–4.5% (w/v). Strain SE56(T) grows lithoautotrophically with H(2) as an electron donor, sulfite or thiosulfate as an electron acceptor, and CO(2)/HCO(3)(−) as a carbon source. It is also capable of chemoorganotrophic growth by reduction of sulfate, sulfite, or thiosulfate. The genome of the new isolate consists of a 2,115,826 bp chromosome with an overall G + C content of 46.0 mol%. The whole-genome annotation confirms the key metabolic features of the novel isolate demonstrated experimentally. Genome contains a complete set of genes involved in CO(2) fixation via reductive acetyl-CoA pathway, gluconeogenesis, hydrogen and fatty acids oxidation, sulfate reduction, and flagellar motility. The phylogenomic reconstruction based on 122 conserved single-copy archaeal proteins supported by average nucleotide identity (ANI), average amino acid identity (AAI), and alignment fraction (AF) values, indicates a polyphyletic origin of the species currently included into the genus Archaeoglobus, warranting its reclassification. Frontiers Media S.A. 2021-07-16 /pmc/articles/PMC8322695/ /pubmed/34335500 http://dx.doi.org/10.3389/fmicb.2021.679245 Text en Copyright © 2021 Slobodkina, Allioux, Merkel, Cambon-Bonavita, Alain, Jebbar and Slobodkin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Slobodkina, Galina
Allioux, Maxime
Merkel, Alexander
Cambon-Bonavita, Marie-Anne
Alain, Karine
Jebbar, Mohamed
Slobodkin, Alexander
Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title_full Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title_fullStr Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title_full_unstemmed Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title_short Physiological and Genomic Characterization of a Hyperthermophilic Archaeon Archaeoglobus neptunius sp. nov. Isolated From a Deep-Sea Hydrothermal Vent Warrants the Reclassification of the Genus Archaeoglobus
title_sort physiological and genomic characterization of a hyperthermophilic archaeon archaeoglobus neptunius sp. nov. isolated from a deep-sea hydrothermal vent warrants the reclassification of the genus archaeoglobus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322695/
https://www.ncbi.nlm.nih.gov/pubmed/34335500
http://dx.doi.org/10.3389/fmicb.2021.679245
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