Cargando…

Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis

Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD)...

Descripción completa

Detalles Bibliográficos
Autores principales: Mei, Chenxue, Wang, Xiao, Meng, Fanxiang, Zhang, Xiaoqing, Chen, Ling, Yan, Siqi, Xue, Junxiu, Sun, Xun, Wang, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322701/
https://www.ncbi.nlm.nih.gov/pubmed/34335262
http://dx.doi.org/10.3389/fphar.2021.696599
_version_ 1783731109273534464
author Mei, Chenxue
Wang, Xiao
Meng, Fanxiang
Zhang, Xiaoqing
Chen, Ling
Yan, Siqi
Xue, Junxiu
Sun, Xun
Wang, Yuanyuan
author_facet Mei, Chenxue
Wang, Xiao
Meng, Fanxiang
Zhang, Xiaoqing
Chen, Ling
Yan, Siqi
Xue, Junxiu
Sun, Xun
Wang, Yuanyuan
author_sort Mei, Chenxue
collection PubMed
description Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17–producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD.
format Online
Article
Text
id pubmed-8322701
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-83227012021-07-31 Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis Mei, Chenxue Wang, Xiao Meng, Fanxiang Zhang, Xiaoqing Chen, Ling Yan, Siqi Xue, Junxiu Sun, Xun Wang, Yuanyuan Front Pharmacol Pharmacology Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17–producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD. Frontiers Media S.A. 2021-07-16 /pmc/articles/PMC8322701/ /pubmed/34335262 http://dx.doi.org/10.3389/fphar.2021.696599 Text en Copyright © 2021 Mei, Wang, Meng, Zhang, Chen, Yan, Xue, Sun and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Mei, Chenxue
Wang, Xiao
Meng, Fanxiang
Zhang, Xiaoqing
Chen, Ling
Yan, Siqi
Xue, Junxiu
Sun, Xun
Wang, Yuanyuan
Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title_full Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title_fullStr Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title_full_unstemmed Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title_short Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
title_sort aucuboside inhibits the generation of th17 cells in mice colitis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322701/
https://www.ncbi.nlm.nih.gov/pubmed/34335262
http://dx.doi.org/10.3389/fphar.2021.696599
work_keys_str_mv AT meichenxue aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT wangxiao aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT mengfanxiang aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT zhangxiaoqing aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT chenling aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT yansiqi aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT xuejunxiu aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT sunxun aucubosideinhibitsthegenerationofth17cellsinmicecolitis
AT wangyuanyuan aucubosideinhibitsthegenerationofth17cellsinmicecolitis