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Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis
Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD)...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322701/ https://www.ncbi.nlm.nih.gov/pubmed/34335262 http://dx.doi.org/10.3389/fphar.2021.696599 |
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author | Mei, Chenxue Wang, Xiao Meng, Fanxiang Zhang, Xiaoqing Chen, Ling Yan, Siqi Xue, Junxiu Sun, Xun Wang, Yuanyuan |
author_facet | Mei, Chenxue Wang, Xiao Meng, Fanxiang Zhang, Xiaoqing Chen, Ling Yan, Siqi Xue, Junxiu Sun, Xun Wang, Yuanyuan |
author_sort | Mei, Chenxue |
collection | PubMed |
description | Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17–producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD. |
format | Online Article Text |
id | pubmed-8322701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83227012021-07-31 Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis Mei, Chenxue Wang, Xiao Meng, Fanxiang Zhang, Xiaoqing Chen, Ling Yan, Siqi Xue, Junxiu Sun, Xun Wang, Yuanyuan Front Pharmacol Pharmacology Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as Rehmannia glutinosa, possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17–producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD. Frontiers Media S.A. 2021-07-16 /pmc/articles/PMC8322701/ /pubmed/34335262 http://dx.doi.org/10.3389/fphar.2021.696599 Text en Copyright © 2021 Mei, Wang, Meng, Zhang, Chen, Yan, Xue, Sun and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Mei, Chenxue Wang, Xiao Meng, Fanxiang Zhang, Xiaoqing Chen, Ling Yan, Siqi Xue, Junxiu Sun, Xun Wang, Yuanyuan Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title | Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title_full | Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title_fullStr | Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title_full_unstemmed | Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title_short | Aucuboside Inhibits the Generation of Th17 Cells in Mice Colitis |
title_sort | aucuboside inhibits the generation of th17 cells in mice colitis |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322701/ https://www.ncbi.nlm.nih.gov/pubmed/34335262 http://dx.doi.org/10.3389/fphar.2021.696599 |
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