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Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase
Isomaltulose is becoming a focus as a functional sweetener for sucrose substitutes; however, isomaltulose production using sucrose as the substrate is not economical. Low-cost feedstocks are needed for their production. In this study, beet molasses (BM) was introduced as the substrate to produce iso...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322766/ https://www.ncbi.nlm.nih.gov/pubmed/34336804 http://dx.doi.org/10.3389/fbioe.2021.691547 |
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author | Wang, Qin-Qing Yang, Ming Hao, Jian-Hua Ma, Zai-Chao |
author_facet | Wang, Qin-Qing Yang, Ming Hao, Jian-Hua Ma, Zai-Chao |
author_sort | Wang, Qin-Qing |
collection | PubMed |
description | Isomaltulose is becoming a focus as a functional sweetener for sucrose substitutes; however, isomaltulose production using sucrose as the substrate is not economical. Low-cost feedstocks are needed for their production. In this study, beet molasses (BM) was introduced as the substrate to produce isomaltulose for the first time. Immobilized sucrose isomerase (SIase) was proved as the most efficient biocatalyst for isomaltulose synthesis from sulfuric acid (H(2)SO(4)) pretreated BM followed by centrifugation for the removal of insoluble matters and reducing viscosity. The effect of different factors on isomaltulose production is investigated. The isomaltulose still achieved a high concentration of 446.4 ± 5.5 g/L (purity of 85.8%) with a yield of 0.94 ± 0.02 g/g under the best conditions (800 g/L pretreated BM, 15 U immobilized SIase/g dosage, 40°C, pH of 5.5, and 10 h) in the eighth batch. Immobilized SIase used in repeated batch reaction showed good reusability to convert pretreated BM into isomaltulose since the sucrose conversion rate remained 97.5% in the same batch and even above 94% after 11 batches. Significant cost reduction of feedstock costs was also confirmed by economic analysis. The findings indicated that this two-step process to produce isomaltulose using low-cost BM and immobilized SIase is feasible. This process has the potential to be effective and promising for industrial production and application of isomaltulose as a functional sweetener for sucrose substitute. |
format | Online Article Text |
id | pubmed-8322766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83227662021-07-31 Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase Wang, Qin-Qing Yang, Ming Hao, Jian-Hua Ma, Zai-Chao Front Bioeng Biotechnol Bioengineering and Biotechnology Isomaltulose is becoming a focus as a functional sweetener for sucrose substitutes; however, isomaltulose production using sucrose as the substrate is not economical. Low-cost feedstocks are needed for their production. In this study, beet molasses (BM) was introduced as the substrate to produce isomaltulose for the first time. Immobilized sucrose isomerase (SIase) was proved as the most efficient biocatalyst for isomaltulose synthesis from sulfuric acid (H(2)SO(4)) pretreated BM followed by centrifugation for the removal of insoluble matters and reducing viscosity. The effect of different factors on isomaltulose production is investigated. The isomaltulose still achieved a high concentration of 446.4 ± 5.5 g/L (purity of 85.8%) with a yield of 0.94 ± 0.02 g/g under the best conditions (800 g/L pretreated BM, 15 U immobilized SIase/g dosage, 40°C, pH of 5.5, and 10 h) in the eighth batch. Immobilized SIase used in repeated batch reaction showed good reusability to convert pretreated BM into isomaltulose since the sucrose conversion rate remained 97.5% in the same batch and even above 94% after 11 batches. Significant cost reduction of feedstock costs was also confirmed by economic analysis. The findings indicated that this two-step process to produce isomaltulose using low-cost BM and immobilized SIase is feasible. This process has the potential to be effective and promising for industrial production and application of isomaltulose as a functional sweetener for sucrose substitute. Frontiers Media S.A. 2021-07-16 /pmc/articles/PMC8322766/ /pubmed/34336804 http://dx.doi.org/10.3389/fbioe.2021.691547 Text en Copyright © 2021 Wang, Yang, Hao and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Wang, Qin-Qing Yang, Ming Hao, Jian-Hua Ma, Zai-Chao Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title | Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title_full | Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title_fullStr | Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title_full_unstemmed | Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title_short | Direct Isomaltulose Synthesis From Beet Molasses by Immobilized Sucrose Isomerase |
title_sort | direct isomaltulose synthesis from beet molasses by immobilized sucrose isomerase |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322766/ https://www.ncbi.nlm.nih.gov/pubmed/34336804 http://dx.doi.org/10.3389/fbioe.2021.691547 |
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