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Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles

[Image: see text] A minimal synthetic cell should contain a substrate for information storage and have the capability to divide. Notable efforts were made to assemble functional synthetic cells from the bottom up, however often lacking the capability to reproduce. Here, we develop a mechanism to ful...

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Autores principales: Dreher, Yannik, Jahnke, Kevin, Schröter, Martin, Göpfrich, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323123/
https://www.ncbi.nlm.nih.gov/pubmed/34251204
http://dx.doi.org/10.1021/acs.nanolett.1c00822
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author Dreher, Yannik
Jahnke, Kevin
Schröter, Martin
Göpfrich, Kerstin
author_facet Dreher, Yannik
Jahnke, Kevin
Schröter, Martin
Göpfrich, Kerstin
author_sort Dreher, Yannik
collection PubMed
description [Image: see text] A minimal synthetic cell should contain a substrate for information storage and have the capability to divide. Notable efforts were made to assemble functional synthetic cells from the bottom up, however often lacking the capability to reproduce. Here, we develop a mechanism to fully control reversible cargo loading and division of DNA-containing giant unilamellar vesicles (GUVs) with light. We make use of the photosensitizer Chlorin e6 (Ce6) which self-assembles into lipid bilayers and leads to local lipid peroxidation upon illumination. On the time scale of minutes, illumination induces the formation of transient pores, which we exploit for cargo encapsulation or controlled release. In combination with osmosis, complete division of two daughter GUVs can be triggered within seconds of illumination due to a spontaneous curvature increase. We ultimately demonstrate the division of a selected DNA-containing GUV with full spatiotemporal control—proving the relevance of the division mechanism for bottom-up synthetic biology.
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spelling pubmed-83231232021-08-02 Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles Dreher, Yannik Jahnke, Kevin Schröter, Martin Göpfrich, Kerstin Nano Lett [Image: see text] A minimal synthetic cell should contain a substrate for information storage and have the capability to divide. Notable efforts were made to assemble functional synthetic cells from the bottom up, however often lacking the capability to reproduce. Here, we develop a mechanism to fully control reversible cargo loading and division of DNA-containing giant unilamellar vesicles (GUVs) with light. We make use of the photosensitizer Chlorin e6 (Ce6) which self-assembles into lipid bilayers and leads to local lipid peroxidation upon illumination. On the time scale of minutes, illumination induces the formation of transient pores, which we exploit for cargo encapsulation or controlled release. In combination with osmosis, complete division of two daughter GUVs can be triggered within seconds of illumination due to a spontaneous curvature increase. We ultimately demonstrate the division of a selected DNA-containing GUV with full spatiotemporal control—proving the relevance of the division mechanism for bottom-up synthetic biology. American Chemical Society 2021-07-12 2021-07-28 /pmc/articles/PMC8323123/ /pubmed/34251204 http://dx.doi.org/10.1021/acs.nanolett.1c00822 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Dreher, Yannik
Jahnke, Kevin
Schröter, Martin
Göpfrich, Kerstin
Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title_full Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title_fullStr Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title_full_unstemmed Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title_short Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles
title_sort light-triggered cargo loading and division of dna-containing giant unilamellar lipid vesicles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323123/
https://www.ncbi.nlm.nih.gov/pubmed/34251204
http://dx.doi.org/10.1021/acs.nanolett.1c00822
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