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Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats

Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the...

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Autores principales: Li, Jun, Jiang, Yinghong, Yu, Hua, Liu, Lejiang, Wang, Qiang, Ju, Hongpin, Zhang, Xuemei, Wang, Wenqi, Yin, Xudong, Wu, Qiong, Xiao, Jianzhong, Miao, Jianrong, Ye, Xiao, Li, Tianyu, Tian, Hui, Xue, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323445/
https://www.ncbi.nlm.nih.gov/pubmed/34318709
http://dx.doi.org/10.1177/09636897211025230
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author Li, Jun
Jiang, Yinghong
Yu, Hua
Liu, Lejiang
Wang, Qiang
Ju, Hongpin
Zhang, Xuemei
Wang, Wenqi
Yin, Xudong
Wu, Qiong
Xiao, Jianzhong
Miao, Jianrong
Ye, Xiao
Li, Tianyu
Tian, Hui
Xue, Wei
author_facet Li, Jun
Jiang, Yinghong
Yu, Hua
Liu, Lejiang
Wang, Qiang
Ju, Hongpin
Zhang, Xuemei
Wang, Wenqi
Yin, Xudong
Wu, Qiong
Xiao, Jianzhong
Miao, Jianrong
Ye, Xiao
Li, Tianyu
Tian, Hui
Xue, Wei
author_sort Li, Jun
collection PubMed
description Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the tail vein or peritoneal cavity of a rat model of radiation enteritis. The temporary protective effect was assessed by identification of donor cells, detection of cellular immune parameters and inflammatory cytokine levels, quantitation of jejunum mucosal preservation and examination of the rat remaining life. Only the rats in the intraperitoneal injection group exhibited a few positive donor cells 7 days after transplantation. CD(4) (+)/CD(8) (+) T cells, a cellular immune parameter, decreased in the abdominal exudate of intraperitoneal injection group, compared with the model-only control and tail vein groups (both P < .05). Both serum and abdominal exudate TNF-α and IL-6 levels in the intraperitoneally injected rats rapidly decreased and were significantly different from those in the model-only control and tail vein injection groups (all P < .05). Mucosal surface area and survival time increased in the intraperitoneal injection group compared with the vehicle and tail vein injection groups (all P = .000). Therefore, the administration of UCMSCs with intraperitoneal injection approach postponed death in a rat model of radiation enteritis, which was associated with reduced serum levels of proinflammatory cytokines (TNF-α, IL-6). However, UCMSCs injected via the tail vein triggered an intense cellular immune response in the serum that adversely affects their survival. This treatment failed to suppress circulating serum and abdominal exudate levels of TNF-α and IL-6 and could not provide a therapeutic benefit for prolonging life against acute radiation enteritis.
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spelling pubmed-83234452021-08-09 Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats Li, Jun Jiang, Yinghong Yu, Hua Liu, Lejiang Wang, Qiang Ju, Hongpin Zhang, Xuemei Wang, Wenqi Yin, Xudong Wu, Qiong Xiao, Jianzhong Miao, Jianrong Ye, Xiao Li, Tianyu Tian, Hui Xue, Wei Cell Transplant Original Article Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the tail vein or peritoneal cavity of a rat model of radiation enteritis. The temporary protective effect was assessed by identification of donor cells, detection of cellular immune parameters and inflammatory cytokine levels, quantitation of jejunum mucosal preservation and examination of the rat remaining life. Only the rats in the intraperitoneal injection group exhibited a few positive donor cells 7 days after transplantation. CD(4) (+)/CD(8) (+) T cells, a cellular immune parameter, decreased in the abdominal exudate of intraperitoneal injection group, compared with the model-only control and tail vein groups (both P < .05). Both serum and abdominal exudate TNF-α and IL-6 levels in the intraperitoneally injected rats rapidly decreased and were significantly different from those in the model-only control and tail vein injection groups (all P < .05). Mucosal surface area and survival time increased in the intraperitoneal injection group compared with the vehicle and tail vein injection groups (all P = .000). Therefore, the administration of UCMSCs with intraperitoneal injection approach postponed death in a rat model of radiation enteritis, which was associated with reduced serum levels of proinflammatory cytokines (TNF-α, IL-6). However, UCMSCs injected via the tail vein triggered an intense cellular immune response in the serum that adversely affects their survival. This treatment failed to suppress circulating serum and abdominal exudate levels of TNF-α and IL-6 and could not provide a therapeutic benefit for prolonging life against acute radiation enteritis. SAGE Publications 2021-07-28 /pmc/articles/PMC8323445/ /pubmed/34318709 http://dx.doi.org/10.1177/09636897211025230 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Li, Jun
Jiang, Yinghong
Yu, Hua
Liu, Lejiang
Wang, Qiang
Ju, Hongpin
Zhang, Xuemei
Wang, Wenqi
Yin, Xudong
Wu, Qiong
Xiao, Jianzhong
Miao, Jianrong
Ye, Xiao
Li, Tianyu
Tian, Hui
Xue, Wei
Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title_full Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title_fullStr Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title_full_unstemmed Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title_short Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
title_sort effects of ucmscs delivered through different transplantation approaches on acute radiation enteritis in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323445/
https://www.ncbi.nlm.nih.gov/pubmed/34318709
http://dx.doi.org/10.1177/09636897211025230
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