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Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats
Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323445/ https://www.ncbi.nlm.nih.gov/pubmed/34318709 http://dx.doi.org/10.1177/09636897211025230 |
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author | Li, Jun Jiang, Yinghong Yu, Hua Liu, Lejiang Wang, Qiang Ju, Hongpin Zhang, Xuemei Wang, Wenqi Yin, Xudong Wu, Qiong Xiao, Jianzhong Miao, Jianrong Ye, Xiao Li, Tianyu Tian, Hui Xue, Wei |
author_facet | Li, Jun Jiang, Yinghong Yu, Hua Liu, Lejiang Wang, Qiang Ju, Hongpin Zhang, Xuemei Wang, Wenqi Yin, Xudong Wu, Qiong Xiao, Jianzhong Miao, Jianrong Ye, Xiao Li, Tianyu Tian, Hui Xue, Wei |
author_sort | Li, Jun |
collection | PubMed |
description | Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the tail vein or peritoneal cavity of a rat model of radiation enteritis. The temporary protective effect was assessed by identification of donor cells, detection of cellular immune parameters and inflammatory cytokine levels, quantitation of jejunum mucosal preservation and examination of the rat remaining life. Only the rats in the intraperitoneal injection group exhibited a few positive donor cells 7 days after transplantation. CD(4) (+)/CD(8) (+) T cells, a cellular immune parameter, decreased in the abdominal exudate of intraperitoneal injection group, compared with the model-only control and tail vein groups (both P < .05). Both serum and abdominal exudate TNF-α and IL-6 levels in the intraperitoneally injected rats rapidly decreased and were significantly different from those in the model-only control and tail vein injection groups (all P < .05). Mucosal surface area and survival time increased in the intraperitoneal injection group compared with the vehicle and tail vein injection groups (all P = .000). Therefore, the administration of UCMSCs with intraperitoneal injection approach postponed death in a rat model of radiation enteritis, which was associated with reduced serum levels of proinflammatory cytokines (TNF-α, IL-6). However, UCMSCs injected via the tail vein triggered an intense cellular immune response in the serum that adversely affects their survival. This treatment failed to suppress circulating serum and abdominal exudate levels of TNF-α and IL-6 and could not provide a therapeutic benefit for prolonging life against acute radiation enteritis. |
format | Online Article Text |
id | pubmed-8323445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-83234452021-08-09 Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats Li, Jun Jiang, Yinghong Yu, Hua Liu, Lejiang Wang, Qiang Ju, Hongpin Zhang, Xuemei Wang, Wenqi Yin, Xudong Wu, Qiong Xiao, Jianzhong Miao, Jianrong Ye, Xiao Li, Tianyu Tian, Hui Xue, Wei Cell Transplant Original Article Radiation enteritis is the most common and serious complication of abdominal or pelvic radiation therapy. Mesenchymal stem cells (MSCs), as well as cell protection agents, inhibit apoptosis and promote the proliferation of injured tissues. 3 human umbilical cords MSCs (UCMSCs) were injected into the tail vein or peritoneal cavity of a rat model of radiation enteritis. The temporary protective effect was assessed by identification of donor cells, detection of cellular immune parameters and inflammatory cytokine levels, quantitation of jejunum mucosal preservation and examination of the rat remaining life. Only the rats in the intraperitoneal injection group exhibited a few positive donor cells 7 days after transplantation. CD(4) (+)/CD(8) (+) T cells, a cellular immune parameter, decreased in the abdominal exudate of intraperitoneal injection group, compared with the model-only control and tail vein groups (both P < .05). Both serum and abdominal exudate TNF-α and IL-6 levels in the intraperitoneally injected rats rapidly decreased and were significantly different from those in the model-only control and tail vein injection groups (all P < .05). Mucosal surface area and survival time increased in the intraperitoneal injection group compared with the vehicle and tail vein injection groups (all P = .000). Therefore, the administration of UCMSCs with intraperitoneal injection approach postponed death in a rat model of radiation enteritis, which was associated with reduced serum levels of proinflammatory cytokines (TNF-α, IL-6). However, UCMSCs injected via the tail vein triggered an intense cellular immune response in the serum that adversely affects their survival. This treatment failed to suppress circulating serum and abdominal exudate levels of TNF-α and IL-6 and could not provide a therapeutic benefit for prolonging life against acute radiation enteritis. SAGE Publications 2021-07-28 /pmc/articles/PMC8323445/ /pubmed/34318709 http://dx.doi.org/10.1177/09636897211025230 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Li, Jun Jiang, Yinghong Yu, Hua Liu, Lejiang Wang, Qiang Ju, Hongpin Zhang, Xuemei Wang, Wenqi Yin, Xudong Wu, Qiong Xiao, Jianzhong Miao, Jianrong Ye, Xiao Li, Tianyu Tian, Hui Xue, Wei Effects of UCMSCs Delivered through Different Transplantation Approaches on Acute Radiation Enteritis in Rats |
title | Effects of UCMSCs Delivered through Different Transplantation
Approaches on Acute Radiation Enteritis in Rats |
title_full | Effects of UCMSCs Delivered through Different Transplantation
Approaches on Acute Radiation Enteritis in Rats |
title_fullStr | Effects of UCMSCs Delivered through Different Transplantation
Approaches on Acute Radiation Enteritis in Rats |
title_full_unstemmed | Effects of UCMSCs Delivered through Different Transplantation
Approaches on Acute Radiation Enteritis in Rats |
title_short | Effects of UCMSCs Delivered through Different Transplantation
Approaches on Acute Radiation Enteritis in Rats |
title_sort | effects of ucmscs delivered through different transplantation
approaches on acute radiation enteritis in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323445/ https://www.ncbi.nlm.nih.gov/pubmed/34318709 http://dx.doi.org/10.1177/09636897211025230 |
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